Figure 4.

In vivo and in vitro characterization of SiTPS. GC-MS of hexane extracts from the in vitro assay in which the purified SiTPS was incubated with the substrate FPP (E,E-FPP) and from the in vivo assay with E. coli strains transformed with the plasmids pIRS, pFF and pET_SiTPS. An E. coli strain carrying only the pIRS and pFF plasmids was used as control. Analytical standards of viridiflorol and ledol were used to identify the produced compound. The mass spectra of the produced compounds and the analytical standards are given in the right hand panels.