Figure 3.

Structure of HPV16/18 including the long control region (LCR) and E6. PCR amplicons were set every 30 bp of the HPV genome spanning the LCR and E6 gene. Each amplicon was 140 bp long including 30-mer primers, and the neighboring amplicons overlapped by 110 bp. The mononucleosomal-sized DNA fragments were quantified by real-time PCR using 21 individual primer pairs of HPV16 or 19 individual primer pairs of HPV18.