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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: Diabetologia. 2021 Mar 26;64(7):1674–1689. doi: 10.1007/s00125-021-05431-5

Fig. 4.

Fig. 4

SIRT1 plays an important role in fasting mediated decrease of cholesterol levels in BRECs. (a) TNF-α treatment causes a significant increase in cholesterol levels while FMC (0% FBS, 24 h) prevents this TNF-α -induced increase. (b) This decrease is amplified by administration of the LXRα activator, DMHCA. (c) SIRT1 was reduced by exposure of the BRECs to Sirt1 siRNA. Administration of Sirt1 siRNA prevented serum-induced upregulation of Abca1 and Abcg1 and inhibited the downregulation of the proinflammatory gene, Vcam1. (d) Sirt1 knockdown efficiency. *p<0.05, **p<0.01, ***p<0.001. Data are represented as mean ± SEM. Ctl, control; CycloA, cyclophilin A, Scram Ctl; scrambled Sirt1 siRNA sequence used in 4c and 4d