Figure S1.

Cold-induced GPCR expression in mouse tissues and Gpr3 transcription in β-less mice housed at thermoneutrality, related to Figures 1 and 2

(A) transcriptional regulation of established BAT activating Gs-coupled receptors in BAT during adaptation to cold.

(B) induction of Gs-coupled receptors in brown (left) and subcutaneous (right) white adipose depots during adaptation to cold (non-normalized values from Figure 1B).

(C) tissue panel of cold-induced Gpr3 expression.

(D) in situ hybridization (ISH) of Gpr3 mRNA (red) in scWAT, E, scWAT (high magnification. Dotted arrow: Unilocular adipocyte. Solid arrow: Multilocular adipocyte), and, F, eWAT of thermoneutral-housed or cold-challenged mice. BAT Gpr3 expression in, G, thermoneutral-acclimated β-less mice and wildtype controls.

For all panels, error bars represent ±SEM, p ≤ 0.05=∗, p ≤ 0.01 = ∗∗, p ≤ 0.001 = ∗∗∗, p ≤ 0.0001 = ∗∗∗∗, t test (C) or Bonferroni's multiple comparisons test (A).