. 2021 May 19;13(13):1031–1056. doi: 10.2217/epi-2021-0037

Table 1. . miRNAs significantly altered by maternal separation and the interaction between maternal separation and sex across prefrontal cortex, amygdala and hippocampus.^†.

miRNAs altered by MS
				CPM fold change
	Accession ID	Chromosome location	miRNA	MS: control	p-value	Sig.
Prefrontal cortex	MIMAT0017093	chr2:165605923–165606020(+)	miR-15b-3p	1.58	0.014	^†
	MIMAT0003211	chrX:140117518–140117589(-)	miR-20b-5p	1.46	0.045	^†
	MIMAT0000847	chr10:75050839–75050925(+)	miR-142-5p	1.33	0.003	^§
	MIMAT0000851	chr18:56969907–56969994(-)	miR-145-5p	1.47	0.022	^†
	MIMAT0000876	chr6:136704961–136705057(+)	miR-203a-3p	1.31	0.029	^†
	MIMAT0001547	chrX:158153377–158153467(+)	miR-450a-5p	1.34	0.005	^§
	MIMAT0003121	chr1:215832088–215832160(-)	miR-483-3p	1.70	0.036	^†
Amygdala	MIMAT0017105	chr8:55492542–55492625(-)	miR-34b-3p	1.79	0.010	^‡
	MIMAT0004723	chr8:55492024–55492100(-)	miR-34c-3p	2.06	0.003	^§
	MIMAT0000814	chr8:55492024–55492100(-)	miR-34c-5p	1.83	0.015	^†
	MIMAT0017122	chr11:88129773–88129854(+)	miR-130b-5p	-1.35	0.039	^†
	MIMAT0035734	chr14:30331049–30331131(-)	miR-193b-3p	1.56	0.007	^‡
	MIMAT0000877	chr1:240403000–240403109(+)	miR-204-5p	1.36	0.013	^†
	MIMAT0017286	chr17:71809047–71809128(-)	miR-466b-2-3p	1.39	0.008	^‡
	MIMAT0037262	chr17:72066536–72066617(-)	miR-466b-4-3p	1.39	0.008	^‡
	MIMAT0024852	chr11:86857633–86857705(+)	miR-1306-3p	-1.38	0.047	^†
Hippocampus	MIMAT0001082	chr4:82199056–82199140(-)	miR-196b-5p	2.59	0.048	^†
	MIMAT0000875	chr5:173490144–173490238(-)	miR-200b-3p	1.57	0.028	^†
	MIMAT0017152	chr5:173490144–173490238(-)	miR-200b-5p	2.66	0.011	^†
	MIMAT0000575	experimental	miR-335	-1.36	0.028	^†
	MIMAT0017221	chr6:133877124–133877205(+)	miR-487b-5p	-1.43	0.033	^†
	MIMAT0017211	chr6:133706202–133706285(+)	miR-540-5p	-1.33	0.032	^†
	MIMAT0024853	chr13:44719335–44719401(+)	miR-3473	1.34	0.025	^†

miRNAs altered by the interaction between MS and sex
				CPM fold change
	Accession ID	Chromosome location	miRNA	M MS: M controls	F MS: F controls	p-value	Sig.
Prefrontal cortex	MIMAT0017087	chr11:16052873–16052966(+)	let-7c-1-3p	-1.23	1.27	0.016	^†
	MIMAT0000785	chr2:165606072–165606166(+)	miR-16-5p	1.36	-1.04	0.023	^†
	MIMAT0000800	chr11:79380523–79380608(-)	miR-28-5p	1.34	1.02	0.037	^†
	MIMAT0017146	chr8:117354364–117354454(+)	miR-191a-3p	-1.35	1.05	0.027	^†
	MIMAT0017148	chr1:221634786–221634870(+)	miR-194-3p	-1.33	1.44	0.040	^†
	MIMAT0004741	chr20:3816158–3816267(-)	miR-219a-1-3p	-1.37	1.28	0.025	^†
	MIMAT0003122	chr6:133739916–133739994(+)	miR-370-3p	-1.34	1.03	0.029	^†
	MIMAT0003201	chr6:133884178–133884257(+)	miR-382-5p	-1.34	1.01	0.027	^†
	MIMAT0017303	chr6:133893997–133894072(+)	miR-410-5p	-1.72	1.05	0.015	^†
	MIMAT0017304	chr6:133858849–133858924(+)	miR-411-3p	-1.48	1.04	0.038	^†
	MIMAT0017307	chr6:133715378–133715458(+)	miR-434-5p	-1.54	-1.09	0.015	^†
	MIMAT0003383	chr10:56844977–56845045(+)	miR-497-5p	1.66	-1.05	0.027	^†
	MIMAT0003177	chr6:133892655–133892744(+)	miR-541-5p	-1.31	1.04	0.041	^†
	MIMAT0005325	chr15:46893157–46893235(+), chr15:47057496–47057574(+)	miR-598-3p	-1.36	1.09	0.042	^†
	MIMAT0035731	chr13:77065446–77065507(+)	miR-1843b-3p	-1.23	1.12	0.010	^‡
	MIMAT0035740	chr6:91645144–91645212(-)	miR-3084a-3p	-1.27	1.36	0.035	^†
	MIMAT0017802	chr10:14603134–14603243(-)	miR-3547	1.41	-1.58	0.024	^†
	MIMAT0025070	chr6:133878578–133878700(+)	miR-6331	-1.41	1.09	0.015	^†
Amygdala	MIMAT0017089	chr17:16418221–16418309(+)	let-7f-1-3p	-1.15	1.27	0.014	^†
	MIMAT0003126	chr9:26795649–26795732(+)	miR-133b-3p	1.50	-1.29	0.030	^†
	MIMAT0035739	chr19:43403103–43403169(+)	miR-3084a-1-5p	1.63	-1.39	0.002	^§
	MIMAT0025056	chr17:34746552–34746678(+), chr17:35477422–35477548(+)	miR-6319	1.14	-1.20	0.024	^†
Hippocampus	MIMAT0000778	chr17:16418221–16418309(+), chrX:21583272–21583354(+)	let-7f-5p	-1.11	1.35	0.035	^†
	MIMAT0004707	chr7:66802731–66802815(+)	let-7i-3p	1.17	-1.32	0.026	^†
	MIMAT0035728	experimental	miR-15a-5p	1.23	-1.46	0.024	^†
	MIMAT0000785	chr2:165606072–165606166(+)	miR-16-5p	1.13	-1.38	0.033	^†
	MIMAT0004717	chr1:38238376–38238456(+), chr13:113782057–113782137(+)	miR-29b-5p	1.11	-1.36	0.027	^†
	MIMAT0000815	chr5:167092491–167092592(+)	miR-34a-5p	1.09	-1.48	0.038	^†
	MIMAT0000828	chr15:47964298–47964382(+), chr2:102311603–102311711(+), chr3:176406205–176406291(+)	miR-124-3p	-1.01	-1.42	0.047	^†
	MIMAT0000865	chr8:73030047–73030131(-), chr8:80928165–80928249(+)	miR-190a-5p	1.23	-1.35	0.049	^†
	MIMAT0004736	chr10:67065896–67065981(+)	miR-193a-5p	1.91	1.13	0.046	^†
	MIMAT0000581	chr10:109195251–109195316(-)	miR-338-3p	1.44	-1.15	0.018	^†
	MIMAT0017306	chr8:117354821–117354903(+)	miR-425-3p	1.02	-1.53	0.020	^†
	MIMAT0001626	chr6:133711425–133711538(+)	miR-431	-1.35	1.26	0.037	^†
	MIMAT0017192	chr6:133712334–133712426(+)	miR-433-5p	-1.03	-1.47	0.035	^†
	MIMAT0001633	chr10:65291204–65291275(-)	miR-451-5p	1.02	2.04	0.009	^‡
	MIMAT0012823	chr4:63342943–63343026(+)	miR-490-3p	1.22	-1.45	0.037	^†
	MIMAT0017217	chr6:133699509–133699592(+)	miR-493-5p	-1.26	1.22	0.048	^†
	MIMAT0005287	chr4:21673635–21673710(-)	miR-879-5p	1.36	-1.39	0.006	^‡

For the main effect of MS, CPM fold change is expressed as a ratio of MS over control animals. For the interaction, CPM fold change is expressed as a ratio of MS over control animals in males only (left column) and females only (right column). Positive fold change indicates increased expression in MS, while negative values indicate decreased expression in MS compared with controls.

^†

p < 0.05.

^‡

p ≤ 0.01.

^§

p < 0.005.

CPM: Counts per million; F: Female; M: Male; MS: Maternal separation; Sig.: Significance.

Table 1. . miRNAs significantly altered by maternal separation and the interaction between maternal separation and sex across prefrontal cortex, amygdala and hippocampus.†.

Table 1. . miRNAs significantly altered by maternal separation and the interaction between maternal separation and sex across prefrontal cortex, amygdala and hippocampus.^†.