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. 2021 Jun 18;10:e60660. doi: 10.7554/eLife.60660

Figure 1. Co-expression and purification of linear ubiquitin chain assembly complex (LUBAC) yields high-quality protein.

(A) SDS-PAGE analysis of individually purified LUBAC components. (B) SDS-PAGE analysis of co-expressed and purified LUBAC. (C) Immunoblot analysis of co-purified LUBAC.

Figure 1.

Figure 1—figure supplement 1. Gel filtration analysis of linear ubiquitin chain assembly complex (LUBAC) showing presence of multiple populations with different oligomeric states.

Figure 1—figure supplement 1.

(A) Gel filtration profile of purified LUBAC separated over S200 column. (B) Tandem gel filtration separation of fraction 3 re-run over S200 column. (C) Molecular weight standards separated over S200 column.