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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Biochimie. 2020 Oct 24;180:1–9. doi: 10.1016/j.biochi.2020.10.012

FIGURE 5. Depletion of Snail in C4-2 prostate cancer cells inhibits neurite outgrowth in NS20Y neuronal cells.

FIGURE 5.

C4-2 NS or Snail shRNA cells were utilized to make conditioned media (CM). (A) Subsequently, NS20Y neuronal cells were cultured for 48 hours with CM followed by a quantitative neurite outgrowth assay that stains and quantifies neurite outgrowth. (B) Neuronal cells simultaneously stained for cell viability were quantified. (C) These same cells were also imaged using Zeiss Confocal microscope in 20X magnification. Data are the average + SD of three independent measurements. Data was analyzed by GraphPad Prism, *p< 0.05, **p< 0.01, ***p< 0.001,****p< 0.0001. Scale bars are 100 μm.