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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: BJOG. 2021 Mar 14;128(8):1282–1291. doi: 10.1111/1471-0528.16660

Figure 4. W48R -mutation in the β-propeller area decreases and A251T -mutation in the I-domain increases the adhesion of CR4-integrin to complement component iC3b.

Figure 4.

A. Representative histograms of flow cytometry analysis show an equal expression of CD11c/CD18 between the wt -and mutant-CR4-transfected COS-1 cells. Shaded line – nontransfected cells, solid line – transfected cells.

B. Non-treated or PDBu-activated COS-1 cells transfected with wt-CD11c/CD18 or W48R-CD11c/CD18 or A251T-CD11c/CD18 were allowed to bind to iC3b coated on plastic, and bound cells were detected enzymatically. Cell binding is reported relative to wild-type CD11c/CD18 transfectant binding, the wild-type values defined as 1. Data are from 3–4 independent experiments. Error bars represent SD horizontal lines show Mean. *p < 0.05