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. 2021 Jun 7;595(7866):278–282. doi: 10.1038/s41586-021-03676-z

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© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 5 — a, Table indicating (1) the total number of unique nanobody genes identified from llama and three nanomice phage display libraries following selection for RBD binding; (2) the number of nanobodies enriched at least tenfold after selection; (3) the number of nanobodies with a unique CRD3; and (4) the different clusters of nanobodies that share similar CDR3 s (with no more than 2 amino acid differences). b, Table showing in vitro neutralization results for the six leading nanobodies using the sVNT kit of GenScript. c, Dot plot depicting the extent of enrichment (y axis) and frequency (x axis) of unique nanobodies after RBD selection of llama (left) or nanomouse 1 (right) libraries. Green circles represent nanobodies that block ACE2–RBD interactions in vitro, black circles are nanobodies that do not efficiently block ACE2–RBD interactions, and grey dots represent untested nanobodies. d, Top graph shows protein alignment of the six nanobodies isolated from llama and nanomice immunized with SARS-CoV-2 spike and RBD. Bottom table shows detailed information of the VHH, D and J domains of nanomouse nanobodies. e, Table depicting equilibrium (K_D), association (K_on) and dissociation (K_off) constants obtained for each nanobody as a monomer (black) or trimer (red) form.