Figure 6.

Assessment of single base substitution (SBS) and indel (ID) tumour mutational signatures (TMS) while varying the VAF threshold, for samples L01 (a, b), L21 (c, d), and L34 (e, f), representing a CRC from a carrier of a germline pathogenic variant in the MSH2, MSH6, and MLH1 genes, respectively. In all cases, ID signatures that have previously been associated with MMRd dominate at most VAF thresholds (b, d, f), while relevant SBS signatures are also present but less dominant, particularly at highly stringent settings (a, c, e).