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. 2021 Jun 15;12(3):e00592-21. doi: 10.1128/mBio.00592-21

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Copyright © 2021 Neville et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 5 — Gallein attenuates P. aeruginosa virulence in C. elegans infection models. (A and B) Kaplan-Meier plot of liquid killing (A) and solid killing (B) assays. Where indicated, gallein was added to 100 μM final concentration. (C) Schematic of bacterial accumulation in the nematode gut. (D) Enumeration of P. aeruginosa CFU to quantify bacterial accumulation in the C. elegans gut. (E) Gallein synergizes with antibiotics to potentiate their activity at sub-MIC doses. (A and B) *, P < 0.05; ***, P < 0.001 (Mantel-Cox log rank test, n = 30 worms per condition). (D and E) ns, P > 0.05; *, P < 0.05; **, P < 0.01; ***, P < 0.001 (two-way ANOVA, Tukey’s multiple-comparison test, n = 30 worms per condition).