KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Critical Commercial Assays | ||
| Standard polycarbonate mouse cages with running wheels | Actimetrics | http://actimetrics.com/products/clocklab/ running-wheel-cages/ |
| Deposited Data | ||
| PER2::LUC signal and location data of single-cell SCN imaging | This paper | To be done. |
| Raw data files for wheel-running activity | This paper | To be done. |
| Experimental Models: Cell lines | ||
| JM8.F6, ES cell line (Strain: C57BL/6N) | UT Southwestern Medical Center Transgenic Core | |
| SNL 76/7 fibroblast, feeder | UT Southwestern Medical Center Transgenic Core | |
| Experimental Models: Organisms/Strain | ||
| Mouse: C57BL/6N | NIH, NCI-Frederick maintained in lab |
Stock 01C55 |
| Mouse: B6N-Per2::iLuc | This paper | |
| Mouse: B6J-Bmal1fx | (Johnson et al., 2014) | |
| Mouse: Viptm1(cre)Zjh/J, congenic, Vip-Cre | Josh Huang, similar to #031628 | JAX: 031628 |
| Mouse: B6-AVP-iCre | (Pei et al., 2014) | AVP-iCre |
| Mouse: C57BL/6-Tg(Nms-icre)20Ywa/J | The Jackson Laboratory | JAX: 027205 |
| Recombinant DNA | ||
| Per2::iLuc targeting vector | This Paper | |
| Per2::Luc targeting vector | (Yoo et al., 2004) | |
| Puro.Cre empty vector | Addgene | Plasmid #17408 |
| DNA Plasmid | ||
| pGEX-2T-mLIF | (Mereau et al., 1993) | |
| Antibodies | ||
| VIP/ Vasoactive Intestinal Peptide | ImmunoStar | 20077 |
| AVP/ Vasopressin | ImmunoStar | 20069 |
| AVPR1A/AVP Receptor V1a | Bioss | bs-11598R |
| VIPR2/ VIP Receptor VPAC2 | Abcam | ab28624 |
| Alexa Fluor 488 conjugated goat anti rabbit IgG | Life tech | A-11034 |
| Experimental Models: Imaging Hardware | ||
| 20x 0.75 NA air microscope objective | Nikon | CFI Plan APO VC 20x |
| 20x 1.00 NA water microscope objective | Olympus | XLUMPLFLN-W 20x |
| Immersion medium for water objective | Zeiss | Immersol 2010 |
| Achromatic Doublet tube lens | Thorlabs | AC254–080-A |
| Non-rotating zoom barrel | Thorlabs | SM1NR1 |
| Beam-splitter units | Photometrics Cairn Research | DV2 OptoSplit II |
| Dichroic mirror | Chroma | T565lpxr-UF2 |
| 550nm short-pass filter | Edmundoptics | #84–708 |
| 625nm long-pass filter | Edmundoptics | #84–746 |
| Cooled CCD camera | Spectral Instruments | 850S |
| Intensified CCD camera | Stanford Photonics | XR/MEGA-10Z |
| Software and Algorithms | ||
| ClockLab USB Data Collection Program (v.3.15) | Actimetrics, Inc. | http://actimetrics.com/downloads/clocklab/ |
| ClockLab Analysis (v.6.0.34 – standalone) | Actimetrics, Inc. | http://actimetrics.com/downloads/clocklab/ |
| Prism (v.8.3) |
GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| SI Image SGL E | Spectral Instruments, Inc. | http://www.specinst.com |
| Piper Control (2.6) | StanfordPhotonics | http://www.stanfordphotonics.com/ |
| ImageJ2 (Fiji) with trackmate | NIH | https://imagej.net/ImageJ2 |
| Python, with minepy, networkx and astropy | Python | https://www.python.org/ |
| R, with multcomp and circular | R | https://www.r-project.org/ |
| Oriana (v.4.0.1) | Kovach Computing Services | https://www.kovcomp.co.uk/oriana/ |