Figure 1.

Inhibiting PDE-7A promotes cell growth of NSCs. A, Nestin-positive cells of NSCs (red). DAPI, nucleus of NSCs (blue). Scale bar, 10 μm. B, C, The silenced efficacy of shPDE7As in mouse NSCs were determined by qRT-PCR (B) and Western blotting (C). D, NSCs infected with shNC, shPDE7A-1, or shPDE7A-2 were cultured for 0, 1, 2, or 4 d, followed by CCK-8 assay. E, F, The overexpressing efficacy of PLVX-PDE7A in mouse NSCs were determined by Western blotting (E), and the OD of PDE7A was analyzed (F). G, NSCs infected with PLVX-PDE7A were cultured for 0, 1, 2, or 4 d, followed by CCK-8 assay; **p < 0.01.