Figure 7.
Evidence for linear arrays of zRGα1a particles on cryo-EM grids
(A) Close-up of a representative micrograph for zRGα1a showing the particle orientation bias by fitting the dominant 2D class average view into picked particles and a recurring linear array of particles highlighted within pale cyan boxes.
(B) Statistical distribution of the difference between the angle psi (Δψ) between two particles and their separation distance. Here the angle ψ is defined for each particle as the angle of rotation of each particle required to align it onto the 2D class average.
(C) 2D class average from automated particle picking containing two adjacent zRGα1a particles.
(D) The zRGα1a-zRGα1a interface highlighted with a black box. The angle and separation between each complex is based on the peak maxima coordinates from (B) assuming both particles are at the same Z height.
(E) An electrostatic potential surface with selected side chains for the homotypic zRETCLD2 interface.
(F) Close-up of the CLD2 contact, highlighting interface residues.
(G) Conservation of representative RET sequences at the CLD2-CLD2 interface shown with an asterix.