Extended Data Fig. 7 |. Competitive, super-stoichiometric recruitment of PUM into RNA-induced droplets.

a, Quantification of the number of PUM1 molecules per PRE in droplets nucleated by NORAD (2 nM) or PRE₈ oligonucleotide (10 nM) and the indicated concentration of PUM1 in vitro. Black line represents the mean (n = 101, 115, 113, 107, 114, or 115 droplets for each condition from left to right). b, Quantification of PUM1 partition coefficients formed with 150 nM PUM1 and 10 nM PRE₈ RNA normalized to partition coefficient at 0 μM competitor RNA (PRE₁ RNA). IC₅₀ represents concentration of PRE₁ RNA needed to reduce PUM1 partitioning by 50%. Data shown as mean ± SD. n = 44 or more droplets analyzed for each data point. Each PRE in the PRE₈ RNA is ~9 times more efficient at PUM1 recruitment than a monovalent PRE.