Maturation conditions, post-ovulatory age, medium pH, and ER stress affect [Ca²⁺]i oscillation patterns in mouse oocytes

Abstract

Insufficiency of oocyte activation impairs the subsequent embryo development in assisted reproductive technology (ART). Intracellular Ca²⁺ concentration ([Ca²⁺]i) oscillations switch the oocytes to resume the second meiosis and initiate embryonic development. However, the [Ca²⁺]i oscillation patterns in oocytes are poorly characterized. In this study, we investigated the effects of various factors, such as the oocytes age, pH, cumulus cells, in vitro or in vivo maturation, and ER stress on [Ca²⁺]i oscillation patterns and pronuclear formation after parthenogenetic activation of mouse oocytes. Our results showed that the oocytes released to the oviduct at 17 h post-human chorionic gonadotrophin (hCG) displayed a significantly stronger [Ca²⁺]i oscillation, including higher frequency, shorter cycle, and higher peak, compared with oocytes collected at earlier or later time points. [Ca²⁺]i oscillations in acidic conditions (pH 6.4 and 6.6) were significantly weaker than those in neutral and mildly alkaline conditions (pH from 6.8 to 7.6). In vitro-matured oocytes showed reduced frequency and peak of [Ca²⁺]i oscillations compared with those matured in vivo. In vitro-matured oocytes from the cumulus-oocyte complexes (COCs) showed a significantly higher frequency, shorter cycle, and higher peak compared with the denuded oocytes (DOs). Finally, endoplasmic reticulum stress (ER stress) severely affected the parameters of [Ca²⁺]i oscillations, including elongated cycles and lower frequency. The pronuclear (PN) rate of oocytes after parthenogenetic activation was correlated with [Ca²⁺]i oscillation pattern, decreasing with oocyte aging, cumulus removal, acidic pH, and increasing ER stress. These results provide fundamental but critical information for the mechanism of how these factors affect oocyte activation.

Supplementary Information

The online version contains supplementary material available at 10.1007/s10815-021-02100-9.

Introduction

Approximately 9–15% of couples worldwide suffer from infertility, and the number is rising steadily [1]. Human assisted reproductive technology (ART) can help infertile couples [2]. Intracytoplasmic sperm injection (ICSI) is the most effective technique used for patients with failed fertilization after conventional in vitro fertilization (IVF) [3]. However, fertilization failure after ICSI still occurs in many cases [4]. Oocyte activation failure is one of the primary reasons for the poor fertilization after ICSI [5]. Assisted oocyte activation is often used to treat patients with low ICSI fertilization rate or fertilization failure [6]. Thus, it is critical to understand the factors affecting the activation process of oocytes.

Under natural conditions, the oocyte is activated by sperm, which further triggers the periodical fluctuation of intracellular calcium concentration ([Ca²⁺]i) [7]. It is found that the enhanced [Ca²⁺]i oscillations are closely associated with the activation of oocytes. For example, downregulation of mitogen-activated protein kinase and pronucleus formation is highly correlated with the frequency of [Ca²⁺]i oscillations [8–10]. In addition, a study by Yazawa et al. on the measurements of Ca²⁺ oscillations revealed that repetitive spike-shaped [Ca²⁺]_i increases in response to oocyte activation with intervals at 2–10 min [11]. Intracellular Ca²⁺ oscillations are mediated by inositol 1,4,5-trisphosphate (IP3) [12] and protein kinase C (PKC), decreasing the level of the maturation promoting factor (MPF) for the resumption of meiosis [13] and initiation of embryonic development. [Ca²⁺]i oscillations affect oocyte activation and subsequent developmental potential of embryos [14]. As reported by Ducibella et al. [14], cellular events depend on the level or duration of an [Ca²⁺]i-dependent enzyme activity. Ozil et al. [8] also revealed that the process of oocyte activation by Ca²⁺ stimulation determines several developmental events such as pronuclear formation, embryo compaction, and blastocyst formation. Clinically, when the oocyte is insufficiently activated during ICSI, assisted oocyte activation can be used to artificially activate the oocytes by facilitating the influx of Ca²⁺, improving reproductive outcomes [8]. The importance of Ca²⁺ activity in oocyte activation and embryo development provides a promising therapeutic possibility to rescue failed fertilization after ICSI. Oocytes can be activated artificially in a variety of ways. There are physical and chemical methods for parthenogenetic activation of mouse oocytes. Physical methods include electrical, temperature, and mechanical stimulation. The most widely used method is electrical stimulation that is simple, easy, and controllable. Ozil et al. [8] studied the relationship between an electric pulse and oocyte activation by controlling the electric pulse’s intensity and frequency. Chemical methods [15] such as ion modulators and protein synthesis inhibitors, osmotic pressure stimulation were also used. The parthenogenetic activation method used in this experiment is to activate mouse oocytes with strontium chloride (SrCl₂). We observed the effect of different treatments on the calcium oscillation patterns of oocytes activated via parthenogenetic activation. Strontium ion is a divalent cation similar to calcium ion and is not consumed during cell development. Parthenogenetic activation provides a unique method to study the early development of oocytes during fertilization without the male gamete’s contribution. It can be accomplished by exposing unfertilized oocytes to a strontium-based culture medium to observe the biochemical and morphological changes [16]. By using parthenogenetic activation, we can accurately record the timing of Ca²⁺ changes, while we cannot accurately record these changes when employing ICSI and IVF, which are difficult to make activation of all examined oocytes synchronously. It is, therefore, an ideal in vitro model for studying [Ca²⁺]i oscillations during fertilization.

Many factors affect the efficiency of oocyte activation and embryo development [17]. The age of a post-ovulatory oocyte affects its developmental capacity, and a series of intracellular signaling pathways are involved in the post-hCG injection aging process. Conformational changes in endoplasmic reticulum (ER) and mitochondria occur naturally in aging cells, which impacts their function in supplying ATP for Ca²⁺ pumps in the ER membrane [18, 19]. The ER functions like a factory for protein synthesis and serves as an intracellular Ca²⁺ pool to maintain the [Ca²⁺]i. Glucose-regulated protein 78 (GRP78) and Chop gene expression are two important regulatory factors in ER stress reaction. When ER stress inducers are present, GRP78 will be upregulated to ameliorate the cell stress. Meanwhile, GRP78 dissociates with phosphor-extracellular-signal-regulated kinase (pERK). The free pERK will then be translocated into the nucleus to trigger the expression of Chop gene to induce programmed apoptosis [20, 21]. The production, maturation, and activation of oocytes require proper pH in the environment. Although oocytes can maintain the balance of intracellular pH in response to the change of environment to a certain degree, highly acidic or alkaline conditions significantly increases cell death rate and decreases fertilization efficiency [22]. This evidence suggests that oocytes are particularly sensitive to the pH in the environment. A study demonstrates that oocytes tightly attaching to cumulus cells can promote the quality of matured oocytes, which will further improve the fertilization and embryo development [23].

Nevertheless, whether and how [Ca²⁺]i oscillations are affected by these factors during maturation and activation of oocytes remains mostly unknown. In our study, considering the limited availability of human oocytes, we utilized parthenogenetic mouse oocytes to perform systematic recordings of the [Ca2+]i oscillation patterns in different conditions mentioned above. We found that [Ca2+]I oscillation patterns changed in oocytes in a time-dependent manner during development. The strongest [Ca2+]i oscillation pattern occurred at 17 h post-hCG injection. Furthermore, multiple factors, including culture medium, oocyte age, pH change, oocyte maturation in vivo or in vitro, presence of cumulus cells, and ER stress, can impact [Ca2+]i oscillation patterns during oocyte activation. These results provide fundamental, and critical information to better understand how these factors affect oocyte activation and the percentage of pronuclei formed, which could positively correlate with the subsequent embryo development [24].

Materials and methods

Animals

ICR mice (6–8 weeks old; female; Beijing Vital River Laboratory Animal Technology Co., Ltd., Beijing, China) were maintained under a 12/12-h light/dark cycle with standard mouse diet and water at the laboratory animal center of the institute.

Oocyte collection and in vitro maturation

All oocytes collected from mouse oviducts were incubated at 37 °C with 5% CO2 and 95% air. For studying the effects of post-ovulatory age of oocyte on [Ca²⁺]i oscillation patterns, mice were super-ovulated by injecting 10 IU of pregnant mare serum gonadotropin (PMSG, Ningbo Hormone Product Co. Ltd., Cixi, China), and 44 h later 10 IU of hCG (Ningbo Hormone Product Co. Ltd., Cixi, China). Five groups of ovulated COCs were collected from oviducts by blunt dissection at 13, 15, 17, 19, and 21 h after the hCG administration. The COCs were denuded in 1 mg/ml hyaluronidase (Sigma-Aldrich, USA). Twenty-five to 30 oocytes were distributed in each experimental group described below.

For studying the effects of surrounding cumulus presence on [Ca²⁺]i oscillation patterns during in vitro oocyte maturation, the COCs and denuded oocytes were collected from mice stimulated with 10 IU PMSG after 44 h. The COCs and denuded germinal vesicle (GV) oocytes were cultured in M2 culture medium (M7167, Sigma-Aldrich, USA) covered with sterile mineral oil (M8410, Sigma-Aldrich, USA) for 17 h. The COCs were then denuded in 1 mg/ml hyaluronidase before parthenogenetic activation.

To study the effect of ER stress on [Ca²⁺]i oscillation patterns, female mice were intraperitoneally injected with 10 IU of PMSG followed by 10 IU hCG treatment 44 h later. After 15.5 h, the mice were sacrificed by cervical dislocation, and the oocytes were collected following the identical procedure described above and discharged into M2 solution. The ER stress inducer tunicamycin (TM, Greensky, China) at concentrations of 0, 2.5, 5, 10, 20, 30, and 50 μg/ml dissolved in M2 medium was added to M2 medium in which oocytes were cultured for 1.5 h before parthenogenetic activation. Control groups received an equal volume of M2 medium treatment.

To study the effect of gradient pH medium on [Ca²⁺]i oscillation patterns, oocytes collected 17 h post-hCG treatment were placed in SrCl₂ medium (10025-70-4, Sangon Biotech, Shanghai) in Ca²⁺-free Chatot Ziomek Bavister (CZB) medium with the pH adjusted to pH 6.8, pH 7.0, pH 7.2, pH 7.4, and pH 7.6 by NaOH and HCl. The pH was monitored with a pH meter (Horiba, B-212, Kyoto, Japan). Control groups were treated with an equal amount of Ca²⁺ -free CZB medium.

Parthenogenetic activation

To induce parthenogenetic activation, the oocytes were incubated in 10 mM SrCl₂ for 3.5 h activation. The oocytes were then cultured in potassium simplex optimized medium (KSOM) (Sigma-Aldrich, USA) after activation. After 1 h, the pronucleus (PN) formation ratio was observed.

Real-time [Ca²⁺]i recording

To record Ca²⁺ changes in cultured cells, Fluo-4AM fluorescent agent (2.5 μM, Beyotime) was added to the culture medium to visualize Ca²⁺ signals. Oocyte [Ca²⁺]i oscillation images were obtained every 20 s and captured for up to 3.5 h using a time-lapse confocal laser microscope (UltraVIEW-VoX; Perkin-Elmer Life Sciences, Waltham, MA, USA). The fluorescent signal was collected using an argon laser at 488 nm (Fig. S1). The peak of Ca²⁺ concentration, Ca²⁺ move speed, and each cycle’s duration were analyzed for each group. As shown in Supplemental Figure S1, we measured the maximum fluorescent intensity to obtain the peak value. The relative peak was calculated by subtracting the baseline intensity from the peak. The cycle of [Ca²⁺]i oscillation was defined as the interval between two adjacent peaks. The change of fluorescent intensity every 20 s was used to calculate the Ca²⁺ moving speed. The difference of amplitude between two adjacent peaks was measured to calculate the Δpeak.

RNA isolation and RT-PCR

The GRP78 and Chop genes relative mRNA levels were assessed by quantitative real-time PCR (qRT-PCR). Total RNA was isolated from 100 oocytes using the RNeasy Micro kit (QIAGEN, Valencia, CA) according to the manufacturer’s instruction. RNA was reverse-transcribed with the Fast Quant RT Kit (Tiangen Biotech Co., LTD, Beijing, China). The oligonucleotide sequences of the primers used for qRT-PCR were GRP78 forward (F) 5'-ACT TGG GGA CCA CCT ATT CCT-3' and reverse (R) 5'-ATC GCC AAT CAG ACG CTC C-3', Chop F 5'-CTG GAA GCC TGG TAT GAG GAT-3' and R 5'-CAG GGT CAA GAG TAG TGA AGG T-3', and GAPDH F 5'-CCC CAA TGT GTC CGT CGT G-3' and R 5'-TGC CTG CTT CAC CAC CTT CT-3'. Real-time PCR was carried out using the SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA, USA) and a real-time thermocycler under the following conditions: (1) 3 min at 95 °C; (2) 40 cycles of 15 s at 95 °C, 20 s at 60 °C, and 20 s at 72 °C; and (3) 5 min at 72 °C. The relative gene expression was determined using the 2^ΔΔCt method normalized to GAPDH.

Statistical analysis

All data were analyzed using SPSS V22.0 software (IBM, Armonk, NY, USA). Data were shown in the format of mean ± standard deviation. The differences in relative and PN rates among all groups were assessed using the chi-square test. One-way ANOVA was used to analyze the relative expression difference in all groups with the LSD post hoc test. Error bars indicate standard deviations. P-values < 0.05 were considered statistically significant. All experiments were repeated at least three times independently.

Results

[Ca2⁺]i oscillation patterns of oocytes collected at 17 h post-hCG showed a higher frequency and shorter cycle than the other groups

To examine the [Ca²⁺]i oscillation pattern changes in oocytes during the activation of oocytes, we measured the fluorescent intensity of Ca²⁺ signals, which reflects the intracellular Ca²⁺ concentration, cycle frequency, and duration, Ca²⁺ speed, peak, and Δpeak at 13, 15, 17, 19, and 21 h post-hCG after parthenogenetic activation of mouse oocytes. As shown in Fig. 1a, the level of Ca²⁺ concentration gradually increased from 13 to 17 h post-hCG activation, following by a dramatic decrease from 19 to 21 h. Similarly, the measurement of Ca²⁺ move speed was firstly increased to the highest level at 17-h time point, then gradually decreased at the following time points (Fig. 1b). For statistical analyses, we further normalized the values of various parameters including frequency, cycle, maintain, move, peak, and Δpeak in oocytes collected at 13, 15, 19, and 21 h post-hCG to those in oocytes collected at 17 h post-hCG (Fig. 1c). We found that [Ca²⁺]i oscillation patterns of the oocytes collected at 17 h post-hCG showed a higher frequency and shorter cycle than the other groups (P < 0.05) (Fig. 1c). The [Ca²⁺]i oscillations of the 21 h post-hCG group were the weakest among all time points (Fig. 1c), showing as the lowest frequency (0.43 ± 0.04, P < 0.05), longest cycle (1.99 ± 0.22, P < 0.05), and smallest peak (0.57 ± 0.03, P < 0.05). Only a 2-h difference post-hCG caused a significant difference in the frequency and cycle, whereas there was no significant difference in the maintain, move, peak, and Δpeak (Fig. 1c). As shown in Fig. 1c, post-ovulatory aging oocytes collected at 21 h post hCG failed to maintain long-term high-intensity Ca²⁺ oscillations.

Fig. 1.

Intracellular free calcium ([Ca²⁺]i) oscillation patterns after parthenogenetic activation in oocytes collected at different times post-human chorionic gonadotropin (hCG) injection. a, b [Ca²⁺]i oscillation patterns at 13-21-h post-hCG. c Relative [Ca²⁺]i oscillation parameters including “frequency,” “cycle,” “maintain,” “move,” “peak,” and “Δpeak” for statistical analyses; each ratio of different groups was compared with the 17-h group. d Rates of pronucleus (PN) formation of oocytes collected at different post-hCG time points after parthenogenetic activation. N = 25 to 30 for each group. Data represent mean ± SD, and chi-square test and one-way ANOVA are used for statistical analysis. *P < 0.05 versus the 17-h group

To test whether the difference of SrCl₂-induced oscillations observed in mouse oocytes with different post-hCG times affected the PN rate, we recorded the PN rates of the different groups. The highest PN rate was found in the 17 h post-hCG group (93.7 ± 0.2%), while there was no significant difference in the PN rates between the 17 h and 19 h post-hCG (91.3 ± 2.3%, P > 0.05) (Fig. 1d). The PN rate of the 13 h post-hCG group (82.3 ± 1.2%, P < 0.05) and 15 h post-hCG group (87.0 ± 1.9%, P < 0.05) was lower than that of the 17 h post-hCG group. The 21 h post hCG group’s PN rate was also significantly lower compared to the 17 h post-hCG group. Also, some embryos developed abnormally in the 21 h post-hCG group (data not shown). These results show that the [Ca²⁺]i changes followed an inverted U pattern after hCG activation, which matches the time course of pronuclear formation, suggesting that post-ovulatory oocyte age correlates with the intracellular Ca²⁺ activity, which is important for the activation of oocytes.

The [Ca²⁺]i oscillations of the pH 7.2 group showed the highest frequency and shortest cycle

We investigated the effects of pH values on [Ca²⁺]i oscillation patterns in oocytes collected at 17 h post-hCG during parthenogenetic activation. Ca2+ signals were recorded for 3.5 h (Fig. 2a, b) while the pH value was continuously measured by a pH meter, which was confirmed to be within 0.1 of standard error.

Fig. 2.

Intracellular free calcium ([Ca²⁺]i) oscillation patterns during parthenogenetic activation under different pH conditions at 17 h post-hCG. a, b [Ca²⁺]i oscillation patterns after parthenogenetic activation in gradient pH conditions from 6.4 to 7.6. c Relative [Ca2+]i oscillation parameters in gradient pH conditions in parthenogenetic activation medium. Each group was compared with the pH 7.2 group. The [Ca2+]i oscillation parameters, “frequency” and “cycle,” were significantly different between pH 7.2 and pH 6.4 or pH 6.6 groups. d Pronucleus (PN) rate in gradient pH conditions of the parthenogenetic activation system. PN ratios of pH 6.4 and pH 6.6 treatments were significantly lower compared with the other groups. N = 25 to 30 for each group. Data represent mean ± SD, and chi-square test and one-way ANOVA are used for statistical analysis. *P < 0.05 versus pH 7.2 group

The [Ca²⁺]i oscillations of the pH 7.2 group showed the highest frequency and the shortest cycle (Fig. 2c). There were no significant differences among pH 6.8, pH 7.0, pH 7.2, pH 7.4, and pH 7.6 groups (Fig. 2c). The [Ca²⁺]i oscillation patterns of the pH 6.4 (Fig. 2a) and pH 6.6 (Fig. 2c) groups showed significantly lower frequency and longer cycle compared to that of the pH 7.2 group.

We counted the PN formation rates in different pH conditions to explore whether the activation medium’s pH influences the PN formation rate. The PN rates among the groups of pH 6.8, pH 7.0, pH 7.2, pH 7.4, and pH 7.6 were not significantly different (all P > 0.05), ranging from 86.4 to 96.9% (Fig. 2d). The PN rate in the pH 6.4 (64.5 ± 3.0%, P < 0.05) or pH 6.6 (80.1 ± 5.2%, P < 0.05) group was significantly lower compared to that of the pH 7.2 group (Fig. 2d).

The [Ca²⁺]i oscillations of the COCs group showed significantly higher frequency, shorter cycle, and higher peak

We compared the [Ca²⁺]i oscillations after parthenogenetic activation of the oocytes matured in vitro. We explored whether there was a difference in [Ca²⁺]i oscillations between oocytes derived from COCs and DOs matured in vitro and COCs matured in vivo (Fig. 3a, b).

Fig. 3.

Intracellular free calcium ([Ca²⁺]i) oscillation patterns of in vivo oocytes and in vitro matured cumulus-denuded oocytes (DOs) and cumulus-enclosed oocyte complexes (COCs) in vitro-matured for 17 h. a, b [Ca²⁺]i oscillation patterns after parthenogenetic activation. c Relative [Ca²⁺]i oscillation parameters of oocytes matured in vivo or in vitro. The in vivo group was compared with the in vitro groups. d Pronucleus (PN) rate of in vivo and in vitro matured oocytes. N = 25 to 30 for each group. Data represent mean ± SD, and chi-square test and one-way ANOVA are used for statistical analysis. *P < 0.05 versus the control group

The [Ca²⁺]i oscillations of the COCs group showed significantly higher frequency (1.36 ± 0.04, P < 0.05), shorter cycle (0.79 ± 0.02, P < 0.05), and higher peak (1.47 ± 0.04, P < 0.05) than the DOs group (Fig. 3c). Moreover, the PN rate of the COCs group (60.6 ± 1.0%) was significantly higher when compared to the DOs group (46.2 ± 4.7%, P < 0.05), but both in vitro groups had lower PN rates than the in vivo group (Fig. 3d). The frequency and peak of the [Ca²⁺]i oscillations (Fig. 3c) in the in vitro groups were lower than the in vivo group.

[Ca²⁺]i oscillations of the ER stress group showed significantly lower frequency and elongated cycle

To determine whether ER stress was induced by TM in mouse oocytes, qRT-PCR was performed to examine the mRNA level of gene GRP78 and gene Chop in the different groups, two classic ER stress markers. Oocytes treated with TM showed significantly higher mRNA levels of GRP78 and Chop compared with the control group (Supplemental Figure S2). The results indicated that the 10 μg/ml TM group’s oocytes had the highest expression levels of ER stress markers.

We observed that TM treatment affected [Ca²⁺]i oscillations in a dose-dependent manner, including elongated cycles and lower frequency (Fig. 4a–c). The effect became more prominent, with an increase in TM concentrations. Treatment with TM at 5–30 μg/ml severely affected the parameters of [Ca²⁺]i oscillations. In contrast, treatment with 50 μg/ml TM resulted in the near disappearance of [Ca²⁺]i oscillations, and some of the oocytes lysed. Correspondingly, the PN rate linearly decreased with increasing TM concentrations (Fig. 4d).

Fig. 4.

Intracellular free calcium ([Ca²⁺]i) oscillation patterns after parthenogenetic activation of oocytes treated with the endoplasmic reticulum stress inducer tunicamycin (TM). a, b Oocytes were treated with tunicamycin (TM) for 1.5 h. The 0-μM group was the control group, and 2.5, 5, 10, 20, 30, and 50 μg/ml TM were respectively added to the M2 medium. c [Ca²⁺]i oscillation parameters in oocytes treated with gradient TM concentrations ranging from 0 to 50 μg/ml. d The pronucleus (PN) rate of oocytes with ER stress induced by TM. N = 25 to 30 for each group. Data represent mean ± SD, and chi-square test and one-way ANOVA are used for statistical analysis. *P < 0.05 versus the control group

Discussion

Oocyte activation and embryo development are affected by many factors such as culture medium, oocyte age, pH change, and oocyte maturation in vivo or in vitro, the presence/absence of cumulus cells, and environmental stress [17]. A large number of studies have found that the enhanced [Ca²⁺]i oscillations are associated with the activation of oocytes. Whether and how [Ca²⁺]i oscillations are affected by these factors during maturation and activation of oocytes remains mostly unknown. To address this question, we utilized parthenogenetic mouse oocytes in this study to perform systematic recordings of the [Ca²⁺]i oscillation patterns in different conditions mentioned above. We found that [Ca²⁺]i oscillation patterns changed in oocytes in a time-dependent manner during development. The strongest [Ca²⁺]i oscillation pattern appeared 17 h post-hCG injection. Meanwhile, multiple factors, including culture medium, oocyte age, pH change, and oocyte maturation in vivo or in vitro, cumulus cells, and ER stress, can impact the [Ca²⁺]i oscillation during oocyte activation.

Post-ovulatory age and [Ca²⁺]i oscillations pattern in oocytes

An early study by Kubiak et al. [25] found that oocytes collected at 12 h post-hCG were difficult to be activated by SrCl₂, suggesting that oocytes need sufficient time to reach post-ovulatory maturation for subsequent development. Ma et al. [26] reported that the blastocyst rate of oocytes collected at 18 h post-hCG was the highest after being activated by SrCl₂. Recent studies found that the enhanced [Ca²⁺]i oscillations are associated with the activation of oocytes. For example, downregulation of mitogen-activated protein kinase and pronucleus formation is positively correlated with the frequency of [Ca²⁺]i oscillations [8–10]. Our results showed that the oocyte activation capacity and [Ca²⁺]i oscillation pattern was regulated by post-ovulatory time. The [Ca²⁺]i oscillations of fully mature oocytes (17 h group) showed the highest frequency, smallest cycle, and largest peak, suggesting a higher degree of oocytes activation of 17 h post hCG injection, which may further lead to a higher PN rate. The mechanism underlying the higher rate of PN is still unclear. We speculate that it might due to the cytoplasm of the oocytes collected at 17 h post-hCG being fully mature [27]. The Ca²⁺ channel receptors’ sensitivity might be increased in oocytes collected at 17 h post-hCG [28]. Furthermore, the decline of proteins needed for metaphase II arrest might make the Ca²⁺ signal more effective in the fully mature oocytes, and the Ca²⁺ pump of the ER is more frequent in the fully mature oocytes [29].

High rates of cell fragmentation and death are observed in aged oocytes, and some of the surviving embryos exhibit birth defects [30]. Indeed, changes in chromosomal segregation errors and mitochondrial DNA arrangements have been reported in aged oocytes [31]. This study reported a maternal age-related deterioration in [Ca²⁺]i oscillations in oocytes collected 21 h post-hCG. The study suggests that the aged oocytes cannot properly handle Ca²⁺ loading into the cytosol, resulting in a dramatic decrease of Ca²⁺ concentration, reduced [Ca²⁺]i oscillation frequency and Ca²⁺ moving speed, and prolonged cycle. These may further lead to failure to produce periodic [Ca²⁺]i oscillations.

Furthermore, the mitochondrial function is impaired, and ATP gradually decreases due to age-related oxidative stress [32]. As a result, mitochondria provide insufficient energy for Ca²⁺ oscillations and subsequent development. These results might have guiding significance for fertilization time to improve the pregnancy rates of IVF-ET. For patients with repeated failure of oocyte activation, the fertilization rate might be improved by appropriately prolonging the of time window for collecting oocytes post hCG injection. This will allow higher frequency and concentration of intracellular Ca²⁺ during oocyte “capacitation,” improving oocyte activation and fertilization. We should avoid using aging oocytes. Bjercke et al. [33] reported that for some patients with unexplained fertilization failure and poor embryo quality and repeated failure, the cleavage rate and embryo quality of oocyte retrieval at 38 h after hCG injection were higher than that of the 36-h group. This may provide a temporal-scale reference for prolonging the time window to collect oocytes from patients in clinical cases. Nevertheless, a large-scale, prospective, randomized controlled study is still needed to determine the specific limit of prolonged fertilization time.

Acidic pH alters [Ca²⁺]i oscillations pattern

The pH critically influences every biological process at the cellular, tissue, and whole-body level. Maintaining normal pH in an intracellular environment sets the foundation for regulating tissue and whole-body function [30]. It has been reported that the optimal pH of whole embryo culture ranges from 7.5 to 8.2, while in vitro culture medium falls between 7.2 and 7.4 [34]. This evidence suggests that oocytes are particularly sensitive to an acidic environment. By exposing the oocytes to a conditioned medium under different pH conditions, we found that the frequency of [Ca²⁺]i oscillations increased in a neutral or slightly alkaline pH medium, suggesting a mildly alkaline or neutral medium was beneficial to the success of parthenogenetic activation. It has been reported that the elevation of the medium pH may inhibit plasma membrane Ca²⁺-ATPase, which leads to the frequent InsP₃-induced Ca²⁺ transients [35]. It is widely known that an acidic pH can suppress the cell cycle [36]. The mammalian oocytes can maintain pH homeostasis within certain ranges through Na⁺/H⁺ and HCO₃^-/Cl^- exchangers against acidosis and alkalosis, respectively [37]. Therefore, [Ca²⁺]i oscillations and PN rate of oocytes exposed in pH ranging from 6.8 to 7.6 was not significantly changed compared with control group. However, an acidic environment reduces the success of parthenogenetic activation. Our study demonstrates the importance of stable pH values of the activation solution and CO₂ concentration of the incubator during oocyte activation in clinical practice [34, 38]. Too high a concentration of CO₂ in the incubator will reduce the pH value and thus affects the effective oscillation of Ca²⁺ oscillations and oocyte activation [39, 40].

IVM oocytes shows reduced [Ca²⁺]i oscillation capacity

We next explored the difference between oocytes matured in vivo or in vitro. In vitro maturation (IVM) of oocytes is an important option in the clinic. However, there are several disadvantages compared with in vivo maturation, including poor oocyte quality and poor embryo development [35]. A previous study also revealed that in vivo matured oocyte exhibits a specific pattern of calcium oscillations affected by in vitro maturation, in vitro aging, and cryopreservation [41]. Our study alluded that the in vitro matured oocytes’ PN rate was lower compared to that of the ovulated oocytes. The decreased activation was related to the reduced frequency and peak of [Ca²⁺]i oscillations after parthenogenetic activation. This could explain why the average fertilization rate and pregnancy rate of oocytes matured in vitro are reduced clinically [42].

Cumulus cells improve oocyte capacity to produce [Ca²⁺]i oscillations

Cumulus cells play an important role in oocyte maturation. They are important in resuming the meiosis of oocytes [21] and facilitating the development of oocytes through close communication [43]. In this study, the PN rate of the COCs group significantly increased compared to the DOs group. Moreover, the [Ca²⁺]i oscillations of the oocyte in the COCs group showed significantly higher frequency, shorter cycle, and higher peak than the DOs group. The cumulus cells benefit oocyte maturation in vitro, as reported in a previous study [39]. Cumulus cells provide nutrition and directly regulate oocyte metabolism by supplying energy via gap junctions, required for [Ca²⁺]i oscillations in oocytes [40]. If the gap junctions are damaged prematurely, oocytes may develop into poor-quality embryos [21]. Thus, cumulus cells during IVM culture are important for producing normal [Ca²⁺]i oscillations and improving oocyte quality.

ER stress affects [Ca²⁺]i oscillations pattern

Oocytes and embryos in vitro are vulnerable to external stresses such as shearing, temperature changes, altered pH, and higher oxygen pressure [43]. The endoplasmic reticulum is a primary intracellular calcium storage compartment that plays a vital role in maintaining Ca²⁺ homeostasis [44, 45]. IP3Rs expressed on the ER membranes are physically and functionally linked with voltage-dependent anionic channels (VDAC) located on the mitochondria through GRP75 [46]. The IP3R-GRP75-VDAC directly transfer Ca²⁺ between ER and mitochondria to maintain Ca²⁺ homeostasis [47]. It has been reported that TM-induced ER stress caused a disturbance in the Ca²⁺ equilibrium due to a decrease in IP3R-GRP75-VDAC interactions [48]. Our study treated oocytes with TM to induce ER stress and explore the relation between ER stress and [Ca²⁺]i oscillations. We showed that ER stress affected oocyte parthenogenetic activation and embryo development through impairment of mouse oocyte [Ca²⁺]i oscillations. The oocytes with EM stress of varying degrees showed disrupted [Ca²⁺]i oscillations pattern and reduced PN rate after parthenogenetic activation. Therefore, during the ART procedures, reducing ER stress of oocytes could be one of the critical factors for the oocytes’ successful activation.

GRP78 and Chop gene expression are two important regulatory factors in the ER stress reaction. When endoplasmic reticulum stress occurs, the expression of GRP78 will be increased to alleviate ER stress response and maintain ER homeostasis. GRP78 is isolated from pERK, IRE1 (inositol essential enzyme-1), and ATF6 (activating transcription factor 6) under ER stress and binds unfolded folding protein in ER to regulate calcium ion [43]. Therefore, GRP78 is an important regulator in endoplasmic reticulum stress. When external stimulation exceeds ER stress, it activates the apoptosis signal. Multiple studies found that when ER stimulation exceeds the self-regulatory range, Chop gene expression will be significantly increased and transferred to the nucleus, inducing apoptosis [44]. Thus, pERK plays a crucial role in the expression of Chop gene. When the ER stress is prolonged, pERK is isolated from GRP78, resulting in the phosphorylation of eIF2 α (eukaryotic initiation factor 2 α), thus upregulating the expression of Chop gene [21]. The pERK signaling pathway can promote the high expression of Chop gene and reverse the survival signal to apoptosis signal. Simultaneously, after ER stress, other factors can also promote Chop gene activation, thus promoting oocyte apoptosis [21, 49]. In our study, utilizing RT-qPCR, we found a dramatic increase of GRP78 and Chop gene expression in responding to TM treatment at 10 ug/ml and above. The upregulation of GRP78 might function to ameliorate the stress reaction in cells. The elevated Chop expression was likely a consequence of the dissociation of the pERK with GRP78. The free pERK will then be translocated into the nucleus to trigger Chop gene expression to induce programmed apoptosis, a protective physiological process in response to stress.

In conclusion, we found that various factors influence oocyte parthenogenetic activation and embryo development through impairment of mouse oocyte [Ca²⁺]i oscillations. [Ca²⁺]i oscillations of 17-h post-hCG oocytes in a pH of 6.8 to 7.6 showed the highest frequency and the shortest cycle. The presence of cumulus cells is beneficial to producing normal [Ca²⁺]i oscillations. In contrast, the frequency and peak of the [Ca²⁺]i oscillations of oocytes matured in vitro were not as high as in the oocytes matured in vivo. ER stress severely affected the parameters of [Ca²⁺]i oscillations, including elongated cycles and lower frequency. Continuing to develop a better system by optimizing the culture environment via precise control of pH, cumulus cells, oocyte age, and excluding ER stress inducers is essential for a higher success rate of in vitro oocyte parthenogenetic activation. These findings may have important implications for improving human ART.

Funding

This work was sponsored by the National Natural Science Foundation of China (No 81671425 and No 81901477).

Data availability

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Declarations

Ethics approval

The study was approved by the Laboratory Animal Care and Use Committee of the Institute of Zoology, Chinese Academy of Sciences.

Consent to participate

Not applicable.

Consent for publication

Not applicable.

Conflict of interest

The authors declare no competing interests.