FIG 5.

Morphological effects on S. Typhimurium NCTC 13348 under antimicrobial pressure. (A) Representative images of the effect of different antimicrobials on the S. Typhimurium isolate NCTC 13348 in exponential growth phase after 2 h of incubation. Antimicrobials are grouped by similar cellular targets. Bacteria were stained with FM4-64, DAPI, and SYTOX green. Images were acquired on an Opera Phenix using a 63× water immersion lens. (B) Three-dimensional principal-component analysis of the mean and standard deviation values of 94 morphological and intensity properties measured for single bacterial cells in each well. Technical triplicate repeats are shown.