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. Author manuscript; available in PMC: 2022 Mar 9.
Published in final edited form as: Biochemistry. 2021 Feb 23;60(9):725–734. doi: 10.1021/acs.biochem.0c00953

Table 2.

Steady State Kinetic Constants for Cj1441a

fixed substrate variable substrate (mM) kcat
(s−1)		 Km
(μM)		 kcat/Km
(M−1 s−1)
NAD+
(5.0 mM)		 UDP-glucose
(0.05 – 3.0)		 1.10 ± 0.02 210 ± 8.0 5200 ± 180
UDP-glucose (5.0 mM) NAD+
(0.05 – 3.0)		 0.80 ± 0.02 190 ± 12 4200 ± 290
ethanolamine (2.0 mM)
NAD+ (5.0 mM)		 UDP-glucose
(0.05 – 3.0)		 0.90 ± 0.04 300 ± 14 3000 ± 190
ethanolamine-P (2.0 mM)
NAD+ (5.0 mM)		 UDP-glucose
(0.05 – 3.0)		 0.80 ± 0.03 210 ± 11 3800 ± 210
serinol (2.0 mM)
NAD+ (5.0 mM)		 UDP-glucose
(0.05 – 3.0)		 0.80 ± 0.03 230 ± 15 3500 ± 240
serinol phosphate (2.0 mM)
NAD+ (5.0 mM)		 UDP-glucose
(0.05 – 3.0)		 0.80 ± 0.03 200 ± 11 4000 ± 220
a

pH 8.7, 25 °C