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. Author manuscript; available in PMC: 2021 Jul 13.
Published in final edited form as: Biochemistry. 2012 Apr 19;51(17):3634–3641. doi: 10.1021/bi300301a

Figure 6.

Figure 6.

Effect of mutations in ABCG2 on drug efflux function. HEK293 cells with stable expression of ABCG2Myc-WT, ABCG2Myc-TM5*6*, ABCG2Myc-ECL3*, ABCG2Myc-TM5*L3*, ABCG2Myc-L3*TM6*, and vector-transfected control cells (Vec) were subjected to mitoxantrone (MTZ) accumulation analysis using flow cytometry as described in Materials and Methods. The relative accumulation of mitoxantrone was calculated after normalization to the expression level of ABCG2 and then to that of the vector-transfected control from three independent experiments. **p < 0.01.