| Strain, strain background (Mus musculus) |
Tsc1fl/fl
|
Jackson Labs |
(Stock# 005680) |
|
| Strain, strain background (Mus musculus) |
Rosa26fsTRAP |
Jackson Labs |
(Stock# 022367) |
|
| Strain, strain background (Mus musculus) |
L7Cre |
Jackson Labs |
(Stock# 010536) |
|
| Strain, strain background (Mus musculus) |
L7GFP |
Jackson Labs |
(Stock# 004690) |
|
| Antibody |
Anti-GFP (Mouse monoclonal) |
Memorial Sloan Kettering Centre |
HtzGFP-19F7 and HtzGFP-19C8 |
50 µg per IP |
| Antibody |
Anti-DLG3 (PSD93) (Mouse monoclonal) |
Biolegend |
818302 |
(1:1000) |
| Antibody |
Anti-KIF3C (Rabbit polyclonal) |
Proteintech |
144333–1-AP |
(1:800) |
| Antibody |
Anti-CDK16 (PCTAIRE) (Rabbit polyclonal) |
Proteintech |
10102–1-AP |
(1:750) |
| Antibody |
Anti-Rpl28 (Rabbit polyclonal) |
Proteintech |
16649–1-AP |
(1:1000) |
| Antibody |
Anti-Gapdh (Mouse monoclonal) |
ThermoFisher |
AM4300 |
(1:10,000) |
| Antibody |
Anti-B Actin (Mouse Monoclonal) |
Cell Signaling |
3700 |
(1:5000) |
| Antibody |
Anti-GFP (Chicken polyclonal) |
ThermoFisher |
A10262 |
(1:1000) |
| Antibody |
Anti-Shank2 (Rabbit polyclonal) |
ThermoFisher |
PA5-78652 |
(1:500) |
| Peptide, recombinant protein |
Purified Recombinant Biotinylated Protein L |
Pierce |
29997 |
|
| Chemical compound, drug |
Streptavidin MyOne T1 Dynabeads |
ThermoFisher |
65601 |
|
| Chemical compound, drug |
Cycloheximide |
Sigma Aldrich |
01810–1G |
|
| Chemical compound, drug |
RNasin |
Promega |
N2515 |
|
| Chemical compound, drug |
Superasin |
ThermoFisher |
AM2694 |
|
| Chemical compound, drug |
DHPC |
Avanti Polar Lipids |
850306 |
|
| Chemical compound, drug |
TRIzol |
ThermoFisher |
15596026 |
|
| Chemical compound, drug |
Papain |
Worthington |
LS003126 |
|
| Chemical compound, drug |
DNase I |
Worthington |
LK003172 |
|
| Commercial assay or kit |
RNAeasy Minelute kit |
Qiagen |
74204 |
|
| Software, algorithm |
R v4.0.2 |
https://cran.r-project.org/ |
|
|
| Software, algorithm |
Prism v9 |
GraphPad |
|
|
| Software, algorithm |
Python v2.7 |
https://www.python.org/ |
|
|
