KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, Peptides, and Recombinant Proteins | ||
| All-trans retinal | Sigma | Cat#R2500 |
| Tetrodotoxin (TTX) | Tocris | Cat#1078 |
| Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA) | Sigma | Cat#E3889 |
| Plectreurys toxin (PLTX) | Alomone labs | Cat#P510 |
| Thapsigargin | Sigma | Cat#T9033 |
| 2-octanol | Sigma | Cat#74858 |
| Precocene I | Sigma | Cat#195855 |
| Experimental Models: Organisms/Strains | ||
| Drosophila: w*; UAS-GCaMP6s/+; UAS-GCaMP6s/+; OK107-Gal4/+ | Bloomington Drosophila Stock Center | Derived from BDSC_854 |
| Drosophila: w*; UAS-GCaMP6s/+; UAS-GCaMP6s/H24-Gal4 | Bloomington Drosophila Stock Center | Derived from BDSC_51632 |
| Drosophila: w*; UAS-CaMPARI/+; +; OK107-Gal4/+ | Bloomington Drosophila Stock Center | Derived from BDSC_58761 and BDSC_854 |
| Drosophila: w*; UAS-GCaMP6s/MB008B-AD; UAS-GCaMP6s/MB008B-DBD | Bloomington Drosophila Stock Center | Derived from BDSC_68291 |
| Drosophila: w*; UAS-PLTX/UAS-GCaMP6s; UAS-PLTX/UAS-GCaMP6s; OK107-Gal4/+ | Choi et al., 2014 | N/A |
| Drosophila: cacMI02836-FlpStop.ND/Y; UAS-GCaMP6s/+; tub-GAL80ts/UAS-FLPD5; OK107-Gal4/+ | Bloomington Drosophila Stock Center | Derived from BDSC_55805, BDSC_854 and BDSC_67681 |
| Drosophila: cacMI02836-FlpStop.ND/Y; UAS-GCaMP6s/+; tub-GAL80ts/+; OK107-Gal4/+ | Bloomington Drosophila Stock Center | Derived from BDSC_67681 and BDSC_854 |
| Drosophila: cacMI02836-FlpStop.ND/Y; LexAop-GCaMP6s/UAS-FLPL; MB247-lexA/VT30604-Gal4 | Bloomington Drosophila Stock Center | Derived from BDSC_67681 and BDSC_BL62141 |
| Drosophila: cacMI02836-FlpStop.ND/Y; LexAop-GCaMP6s/CyO; MB247-lexA/VT30604-Gal4 | Bloomington Drosophila Stock Center | Derived from BDSC_67681 |
| Drosophila: w*; LexAop-GCaMP6s/+; MB247-lexA, VT30604-Gal4/+ | Vienna Drosophila Resource Center and Bloomington Drosophila Stock Center | Derived from VDRC_100638 |
| Drosophila: w*; LexAop-GCaMP6s/UAS-Met-RNAi, UAS-Gce-RNAi; MB247-lexA, VT30604-Gal4/+ | Vienna Drosophila Resource Center and Bloomington Drosophila Stock Center | Derived from VDRC_100638 and BDSC_61852 |
| Drosophila: w*; UAS-CaMPARI/UAS-Met-RNAi, UAS-Gce-RNAi; +; OK107-Gal4/+ | Vienna Drosophila Resource Center and Bloomington Drosophila Stock Center | Derived from VDRC_100638, BDSC_61852, BDSC_58761, and BDSC_854 |
| Drosophila: w*; UAS-GCaMP6s/+; UAS-GCaMP6s/tub-GAL80ts; OK107-Gal4/+ | Bloomington Drosophila Stock Center | Derived from BDSC_854 |
| Drosophila: w*; UAS-GCaMP6s/UAS-Met-RNAi, UAS-Gce-RNAi; UAS-GCaMP6s/tub-GAL80ts; OK107-Gal4/+ | Vienna Drosophila Resource Center and Bloomington Drosophila Stock Center | Derived from VDRC_100638 and BDSC_61852 and BDSC_854 |
| Drosophila: CantonS | N/A | N/A |
| Drosophila: w*; +; VT30604-Gal4/UAS-gtACR1 | Vienna Drosophila Resource Center and Mohammad et al., 2017 | Derived from VDRC_200228 |
| Drosophila: w*; MB005B-AD/+; MB005B-DBD/UAS-gtACR1 | Bloomington Drosophila Stock Center and Mohammad et al., 2017 | Derived from BDSC_68306 |
| Drosophila: w*; +; UAS-gtACR1(attP2)/+ | Mohammad et al., 2017 | N/A |
| Drosophila: w*; +; UAS-gtACR1(attP2)/+; OK107-Gal4/+ | Bloomington Drosophila Stock Center and Mohammad et al., 2017 | Derived from BDSC_854 |
| Drosophila: w*; c739-Gal4/+; UAS-gtACR1(attP2)/+ | Bloomington Drosophila Stock Center and Mohammad et al., 2017 | Derived from BDSC_7362 |
| Drosophila: w*; MB008B-AD/+; MB008B-DBD/UAS-gtACR1 | Bloomington Drosophila Stock Center and Mohammad et al., 2017 | Derived from BDSC_68291 |
| Drosophila: w*; +; VT44966-Gal4/UAS-gtACR1 | Vienna Drosophila Resource Center and Mohammad et al., 2017 | Derived from VDRC_203571 |
| Drosophila: w*; +; H24-Gal4/UAS-gtACR1 | Bloomington Drosophila Stock Center and Mohammad et al., 2017 | Derived from BDSC_51632 |
| Drosophila: w*; +; VT30604-Gal4/+ | Vienna Drosophila Resource Center | VDRC_200228 |
| Drosophila: w*; UAS-Met-RNAi, UAS-Gce-RNAi/+; + | Vienna Drosophila Resource Center and Bloomington Drosophila Stock Center | Derived from VDRC_100638 and BDSC_61852 |
| Drosophila: w*; UAS-Met-RNAi, UAS-Gce-RNAi/+; VT30604-Gal4/+ | Vienna Drosophila Resource Center and Bloomington Drosophila Stock Center | Derived from VDRC_100638, VDRC_200228 and BDSC_61852 |
| Drosophila: w*; UAS-Met-RNAi, UAS-Gce-RNAi/+; tub-GAL80ts/+ | Vienna Drosophila Resource Center and Bloomington Drosophila Stock Center | Derived from VDRC_100638 and BDSC_61852 |
| Drosophila: w*; UAS-Met-RNAi, UAS-Gce-RNAi/+; VT30604-Gal4/tub-GAL80ts | Vienna Drosophila Resource Center and Bloomington Drosophila Stock Center | Derived from VDRC_100638, VDRC_200228 and BDSC_61852 |
| Drosophila: w*; UAS-GCaMP6s/UAS-gtACR1-mCherry(su(Hw)attP5); UAS-GCaMP6s/+; OK107-Gal4/+ | Vivek Jayaraman and Bloomington Drosophila Stock Center | Derived from BDSC_854 |
| Drosophila: w*; UAS-GCaMP6s/+; UAS-dcr2/+; OK107-Gal4/+ | Bloomington Drosophila Stock Center | Derived from BDSC_854 |
| Drosophila: w*; UAS-GCaMP6s/+; UAS-dcr2/UAS-Itpr-RNAi; OK107-Gal4/+ | Vienna Drosophila Resource Center and Bloomington Drosophila Stock Center | Derived from VDRC_6484 and BDSC_854 |
| Drosophila: w*; UAS-GCaMP6s/+; UAS-dcr2/UAS-RyR-RNAi; OK107-Gal4/+ | Bloomington Drosophila Stock Center | Derived from BDSC_29445 and BDSC_854 |
| Drosophila: w*; UAS-GCaMP6s/UAS-Cac-RNAi; UAS-dcr2/+; OK107-Gal4/+ | Vienna Drosophila Resource Center and Bloomington Drosophila Stock Center | Derived from VDRC_104168 and BDSC_854 |
| Drosophila: w*; UAS-GCaMP6s/+; UAS-dcr2/UAS-Ca-α1D-RNAi; OK107-Gal4/+ | Bloomington Drosophila Stock Center | Derived from BDSC_33413 and BDSC_854 |
| Drosophila: w*; UAS-GCaMP6s/+; UAS-dcr2/UAS-Ca-β-RNAi; OK107-Gal4/+ | Bloomington Drosophila Stock Center | Derived from BDSC_29575 and BDSC_854 |
| Drosophila: w*; UAS-GCaMP6s/+; UAS-dcr2/UAS-Ca-α1D-RNAi; OK107-Gal4/+ | Vienna Drosophila Resource Center | Derived from VDRC_51491 |
| Drosophila: w*; UAS-GCaMP6s/UAS-Ca-β-RNAi; UAS-dcr2/+; OK107-Gal4/+ | Vienna Drosophila Resource Center | Derived from VDRC_v102188 |
| Drosophila: w*; UAS-GCaMP6s/tub-GAL80ts; UAS-GCaMP6s/UAS-SERCA-RNAi; OK107-Gal4/+ | Bloomington Drosophila Stock Center | Derived from BDSC_44581 |
| Drosophila: w*; UAS-GCaMP6s/+; ecd1ts; OK107-Gal4/+ | Bloomington Drosophila Stock Center | Derived from BDSC_218 |
| Drosophila: w*; UAS-GCaMP6s/CyO; UAS-GCaMP6s/TM2; OK107-Gal4/cID | Bloomington Drosophila Stock Center | Derived from BDSC_854 |
| Drosophila: w*; UAS-GCaMP6s/UAS-EcR.B1-DN; UAS-GCaMP6s/+; OK107-Gal4/+ | Bloomington Drosophila Stock Center | Derived from BDSC_6842 |
| Drosophila: w*; UAS-GCaMP6s/UAS-DopEcR-RNAi; UAS-GCaMP6s/+; OK107-Gal4/+ | Vienna Drosophila Resource Center | Derived from VDRC_103494 |
| Drosophila: w*; LexAop-GCaMP6s, UAS-Met-RNAi/MB005B-AD; MB247-lexA/MB005B-DBD | Bloomington Drosophila Stock Center and Vienna Drosophila Resource Center | Derived from VDRC_100638 and BDSC_68306 |
| Drosophila: w*; LexAop-GCaMP6s, UAS-Met-RNAi/+; MB247-lexA/+ | Vienna Drosophila Resource Center | Derived from VDRC_100638 |
| Drosophila: w*; LexAop-GCaMP6s, UAS-Gce-RNAi /MB005B-AD; MB247-lexA/MB005B-DBD | Bloomington Drosophila Stock Center | Derived from BDSC_61852 and BDSC_68306 |
| Drosophila: w*; LexAop-GCaMP6s, UAS-Gce-RNAi/+; MB247-lexA/+ | Bloomington Drosophila Stock Center | Derived from BDSC_61852 |
| Drosophila: Met1/Y; UAS-GCaMP6s/+; UAS-GCaMP6s/+; OK107-Gal4/+ | Bloomington Drosophila Stock Center | Derived from BDSC_3472 |
| Drosophila: w*; UAS-GCaMP6s/+; UAS-GCaMP6s/UAS-Met.ORF; OK107-Gal4/+ | Zurich ORFeome Project | Derived fro FlyORF_F000644 |
| Drosophila: w*; UAS-GCaMP6s/MB005B-AD; UAS-GCaMP6s/MB005B-DBD | Bloomington Drosophila Stock Center | Derived from BDSC_68306 |
| Software and Algorithms | ||
| MATLAB R2016b | The MathWorks | https://www.mathworks.com/products/matlab.html |
| SlideBook | 3i Intelligent Imaging Innovations | https://www.intelligent-imaging.com/ |
| FIJI (ImageJ) | National Institutes of Health | http://fiji.sc/ |
| GraphPad Prism 6 | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |