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[Preprint]. 2021 Jul 5:2021.07.03.450938. [Version 1] doi: 10.1101/2021.07.03.450938

Figure 5. HTRF assay detects NP produced by live virus infection of Vero E6.

Figure 5.

The TR-FRET ratio for SARS-CoV-2 NP detected in Vero E6 infected with the SARS-CoV-2 USA-WA1/2020 strain at different MOI starting at 0.25 and diluted 1:3 based on a reverse time-course protocol (A) for 0 h (B), 12 h (C), 24 h (D), or 48 h (E). The standard curve (F) with assay conditions equal to the 48h time-point. Plates were incubated with NP HTRF reagents for 1h at RT or O/N at 4°C. N=3 wells in a half-area 96-well plate. Error bars indicate S.D.