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[Preprint]. 2021 Jul 5:2021.07.03.450938. [Version 1] doi: 10.1101/2021.07.03.450938

Figure 6. NP HTRF assay confirmation of remdesivir inhibition of SARS-CoV-2 replication.

Figure 6.

The TR-FRET ratio for SARS-CoV-2 NP in Vero E6 infected with SARS-CoV-2 USA-WA1/2020 strain at MOI 0.009 based on protocol (A) for 24 h (B). Remdesivir was added at a starting concentration of 100 μM and serially diluted 1:3. The standard curve (C) using recombinant SARS-CoV-2 NP. Plates were incubated with NP HTRF reagents for 1h at RT or O/N at 4°C. N=3 wells in a half-area 96-well plate. Error bars indicate S.D.