Author response table 1. Antigenicity of HDV particles.

RT-qPCR assay quantifying HDV RNA in the supernatants of producer cells was used to normalize preparations of viral particles prior to being subjected to ELISA specific for the AGL determinant. The results are presented as fold-change relative to that of the wt. GE: HDV RNA genome equivalents; no-GP: supernatants of Huh7 cells transfected with a trimer of HDV cDNA (pSVL-D3) and an empty plasmid; wt-LMS: supernatants of Huh7 cells transfected with pSVL-D3 and HBV wt LMS envelope proteins; Wt-S: HBV S only; LMS(T303C): HBV LMS envelope proteins bearing a T140C mutation; LMS(TG/CC): HBV LMS envelope proteins bearing T140C and G145C mutations.

MUREX	1E4 GE	1E5 GE	CLIA	1E4 GE	1E5 GE
no-GP	neg	neg	no-GP	neg	neg
wt-LMS	1.00	1.00	wt-LMS	1.00	1.00
wt-S	1.20	1.13	wt-S	1.64	1.43
LMS(T303C)	0.91	0.97	LMS(T303C)	neg	neg
LMS(TG/CC)	neg	neg	LMS(TG/CC)	neg	neg