REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-mouse ACKR1 (clone 6B7)	(Thiriot et al., 2017)	N/A
Anti-mouse CD117 (c-Kit) Alexa Fluor 647 (Clone 2B8)	Biolegend	Cat# 105818; RRID: AB_493474
Anti-mouse CD117 (c-Kit) APC (Clone 2B8)	Biolegend	Cat# 123121; RRID: AB_313220
Anti-mouse CD16/32 Purified Antibody	Biolegend	Cat# 101301; RRID: AB_312800
Anti-mouse CD182 (CXCR2) PE (Clone SA044G4)	Biolegend	Cat# 149303; RRID: AB_2565691
Anti-mouse CD184 (CXCR4) Monoclonal Antibody (2B11), PerCP-eFluor 710	Thermo Fischer Scientific	Cat# 46-9991-80; RRID: AB_10670489
Anti-mouse CD31 (clone 390)	Thermo Fischer Scientific	Cat# 16-0311-85; RRID: AB_468933
Anti-mouse CD45 Pacific Blue (clone 30-F11)	Biolegend	Cat# 103126; RRID: AB_493535
Anti-mouse CD54 PE/Cy7 (Clone YN1/1.7.4)	Biolegend	Cat# 116121; RRID: AB_2715949
Anti-mouse CD62L Brilliant Violet 605 (Clone MEL-14)	Biolegend	Cat# 104437; RRID: AB_11125577
Anti-mouse c-Kit (CD117) (clone D13A2)	Cell Signaling Technology	Cat# 3074T; RRID: AB_1147633
Anti-mouse CXCL1 (polyclonal)	R&D systems	Cat# AF-453-NA; RRID: AB_354495
Anti-mouse CXCR2 Alexa Fluor 647 (clone SA044G4)	Biolegend	Cat# 149305; RRID: AB_2565693
Anti-mouse F4/80 Alexa Fluor 647 (Clone BM8)	Biolegend	Cat# 123121; RRID: AB_893492
Anti-mouse FcεRIα Pacific Blue (Clone MAR1)	Biolegend	Cat# 134313; RRID: AB_10612933
Anti-mouse GRK2	Genetex	Cat# GTX101682
Anti-mouse Ly6G Pacific Blue™ (Clone 1A8)	Biolegend	Cat# 127611 RRID: AB_1877212
Anti-mouse Ly6G Alexa Fluor 488 (Clone 1A8)	Biolegend	Cat# 127625; RRID: AB_1186108
Anti-mouse Ly6G Alexa Fluor 647 (clone 1A8)	Biolegend	Cat# 127610; RRID: AB_1134159
Anti-mouse Ly6G Biotin (Clone 1A8)	Biolegend	Cat# 127604; RRID: AB_2561339
Anti-mouse MRP14 (clone 2B10)	Gift from Dr N.Hogg (Francis Crick Institute, UK)	N/A
Anti-mouse/human CD11b Brilliant Violet 711 (Clone M1/70)	Biolegend	Cat# 101241; RRID: AB_11218791
Anti-mouse/rat CD29 PE/Cy7 (Clone HMβ1-1)	Biolegend	Cat# 102221; RRID: AB_528789
Anti-mouse CD206 Alexa Fluor 647 (clone C068C2)	Biolegend	Cat# 141711; AB_10900240
Anti-Neutrophil Elastase antibody	Abcam	Cat# ab68672; RRID: AB_1658868
Armenian Hamster IgG Isotype Ctrl PE/Cy7	Biolegend	Cat# 400921
Blocking anti-mouse CXCL1 (clone 48415)	R&D systems	Cat# MAB453; RRID: AB_2087696
Blocking anti-mouse CXCL2 (clone 40605)	R&D systems	Cat# MAB452; RRID: AB_2230058
Blocking anti-mouse IgG2a	Biolegend	NA
Depletion Anti-mouse CD117 (c-kit) Antibody Ultra-LEAF™ Purified (Clone ACK2)	Biolegend	Cat# 135131; RRID: AB_2571992
Depletion Anti-mouse IgG2b κ Isotype control (Clone ACK2)	Biolegend	Cat# 135131; RRID 2571992
Polyclonal goat anti-rabbit immunoglobulins/HRP conjugated antibody	Agilent/Dako	Cat# P044801-2
Rat IgG2a, κ Isotype Ctrl PE (Clone RTK2758)	Biolegend	Cat# 400507
Rat IgG2b kappa Isotype Control (eB149/10H5), PerCP-eFluor 710	Thermo Fischer Scientific	Cat# 46-4031-80; RRID: AB_1834457
Rat IgG2b, κ Isotype Ctrl Antibody Brilliant Violet 711 (Clone RTK4530)	Biolegend	Cat#400653
Biological Samples
Bone marrow: mouse Mrp8-Cre;Grk2f/f;Lyz2-EGFP-ki	Dr Tim Lämmermann (Max Planck Institute of Immunobiology and Epigenetics, Germany)	N/A
Critical commercial assay
Alexa Fluor 488 antibody labeling kit	Thermo Fisher Scientific	Cat# A20181
Alexa Fluor 555 antibody labeling kit	Thermo Fisher Scientific	Cat# A20187
Alexa Fluor 647 antibody labeling kit	Thermo Fisher Scientific	Cat# A20186
Anti-Ly-6G MicroBeads UltraPure, mouse	Milteny biotech	Cat# 130-120-337
DyLight 405 antibody labeling kit	Thermo Fisher Scientific	Cat# 53021
Mouse CXCL1/KC DuoSet ELISA	R&D Systems	Cat# 453-05
Proteome Profiler Mouse Cytokine Array Kit, Panel A	R&D Systems	Cat# ARY006
Chemicals, Peptides, and Recombinant Proteins
AF647-streptavidin	Thermo Fisher Scientific	Cat# S21374
Avidin, Alexa Fluor 488 conjugate	Thermo Fischer Scientific	Cat# A21370
Avidin, Egg White	Thermo Fisher Scientific	Cat# A2667
Bafilomycin	AlfaAesar	Cat# J67193
BSA, low endotoxin	Sigma-Aldrich	Cat# A9543
C12FDG (5-Dodecanoylaminofluorescein Di-β-D-Galactopyranoside)	Thermo Fischer Scientific	Cat# D2893
DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate)	Biolegend	Cat# 422801
Evans Blue	Sigma	Cat# E2129
FITC Annexin V	BD	Cat# 560931
Halt Protease and Phosphatase Inhibitor Cocktail (100X)	Thermo Fisher Scientific	Cat# 78440
Propidium iodide solution	Biolegend	Cat# 421301
Recombinant murine IL-1β	R&D Systems	Cat# 401-ML-005/CF
Recombinant murine TNF-α aa 80-235	R&D Systems	Cat# 410-MT-010/CF
Triton X-100	Sigma	Cat# T8787-100ML
Experimental Models: Organisms/Strains
Mouse, Ackr1−/−	(Dawson et al., 2000)	N/A
Mouse C57BL/6	Charles River laboratories	JAX 000664
Mouse, C57BL/6JRj	Janvier laboratories	Cat# SC-C57J-M
Mouse, Lyz2-EGFP-ki	Gift from Dr M. Sperandio (Ludwig Maximilians University Munich, Germany) (Faust et al., 2000)	N/A
Mouse, Mcpt5-Cre-R-DTA	Provided by Prof. Axel Roers (Medical Faculty Carl Gustav Carus, Technische Universität Dresden)	N/A
Software and Algorithms
FlowJo v10	Tree Star	https://www.flowjo.com/
ImageJ	Wayne Rasband (NIH)	https://imagej.nih.gov/ij/
Imaris v9	Bitplane	https://imaris.oxinst.com/packages
Prism v8	Graphpad	https://www.graphpad.com/scientific-software/prism/
Other
123 eBeads™ counting beads	Thermo Fisher Scientific	Cat# 01-1234-42
FluoSpheres™; Carboxylate-Modified Microspheres, 0.02 μm, red fluorescent (580/605)	Invitrogen	Cat# F8786
Latex-Free Orthodontic Elastic Bands	Dental Aesthetics	Cat# UNL735-F
UltraComp eBeads™ Compensation Beads	ThermoFisher Scientific	Cat# 01-2222-42
Zombie Yellow™ Fixable Viability Kit	Biolegend	Cat# 423103