Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Mus musculus) | MC3T3-E1 Subclone 14 | The Cell Bank of the Chinese Academy of Sciences | Cat #:GNM15 RRID:CVCL_5437 |
|
| Cell line (Mus musculus) | RAW 264.7 | The Cell Bank of the Chinese Academy of Sciences | Cat #:SCSP-5036 RRID:CVCL_0493 |
|
| Peptide, recombinant protein | BDNF | PeproTech | Cat #:450–02 | |
| Peptide, recombinant protein | RANKL | R and D Systems | Cat #:462-TEC-010 | |
| Chemical compound, drug | 7,8- Dihydroxyflavone; 7,8-DHF | Tokyo Chemical Industry | Cat #:D1916 | |
| Chemical compound, drug | Ascorbic acid | Aladdin | Cat #:A103539 | |
| Chemical compound, drug | β-Glycerophosphate disodium salt hydrate | Sigma-Aldrich | Cat #:G9422 | |
| Chemical compound, drug | Dexamethasone | Sigma-Aldrich | Cat #:D4902 | |
| Chemical compound, drug | K252a | Cell Signaling | Cat #:12754 | |
| Antibody | Rabbit monoclonal anti-GSK3β | Huabio | Cat #:ET1607-71 | WB: 1:1000 |
| Antibody | Rabbit monoclonal anti-p-GSK3β (Ser 9) | Huabio | Cat #:ET1607-60 | WB: 1:1000 |
| Antibody | Rabbit monoclonal anti-β-catenin | Abcam | Cat # ab32572 | WB: 1:5000 |
| Antibody | Rabbit monoclonal anti-Osterix | Abcam | Cat #:ab209484 | WB: 1:1000 |
| Antibody | Rabbit monoclonal anti-Runx2 | Cell Signaling | Cat #:12556 | WB: 1:1000 |
| Antibody | Rabbit monoclonal anti-Smad2 | Huabio | Cat #:ET1604-22 | WB: 1:1000 |
| Antibody | Rabbit monoclonal anti-p-Smad2 (S250) | Huabio | Cat #:ET1612-32 | WB: 1:1000 |
| Antibody | Rabbit monoclonal anti-TAK1 | Huabio | Cat #:ET1705-14 | WB: 1:1000 |
| Antibody | Rabbit polyclonal anti-TrkB | Affinity | Cat #:AF6461 | WB: 1:1000 |
| Antibody | Rabbit polyclonal anti-P-TrkB (Tyr706) | Affinity | Cat #:AF3461 | WB: 1:1000 |
| Antibody | Rabbit monoclonal anti-MMP-9 | Abcam | Cat #:ab228402 | WB: 1:1000 |
| Antibody | Rabbit polyclonal anti-Adamts5 | Huabio | Cat #:1903–32 | WB: 1:1000 |
| Antibody | Rabbit polyclonal anti-GAPDH | Proteintech | Cat #:10494–1-AP | WB: 1:5000 |
| Sequence-based reagent | qRT-PCR primers | This paper | See Supplementary file 1 | |
| Sequence-based reagent | siRNA | This paper | See Materials and methods, Small interfering RNA transfection section | |
| Commercial assay or kit | Cell Counting Kit-8; CCK-8 | Biosharp | Cat #:BS350A | |
| Commercial assay or kit | DNA Content Quantitation Assay (Cell Cycle) kit | Solarbio | Cat #:CA1510 | |
| Commercial assay or kit | BCA Protein Assay Kit | Solarbio | Cat #:PC0020 | |
| Commercial assay or kit | ALP assay kit | Nanjing Jiancheng | Cat #:A059-2 | |
| Commercial assay or kit | Alizarin Red S solution (1%, pH 4.2) | Solarbio | Cat #:G1452 | |
| Commercial assay or kit | Acid phosphatase, leukocyte (TRAP) kit | Sigma-Aldrich | Cat #:387A | |
| Commercial assay or kit | TRIzol Reagent | Invitrogen | Cat #:15596026 | |
| Commercial assay or kit | PrimeScript RT reagent Kit with gDNA Eraser | TaKara | Cat #:RR047A | |
| Commercial assay or kit | PowerUp SYBR Green Master Mix | Applied Biosystems | Cat #:A25742 | |
| Commercial assay or kit | Lipofectamine 3000 | Invitrogen | Cat #:L3000001 | |
| Commercial assay or kit | Rat FSH ELISA kit | Cusabio | Cat #:CSB-E06869r | |
| Commercial assay or kit | Rat E2 ELISA kit | Cusabio | Cat #:CSB-E05110r | |
| Commercial assay or kit | Rat BGP ELISA kit | Cusabio | Cat #:CSB-E05129r | |
| Software, algorithm | ModFit LT | Verity Software House (http://www.vsh.com/products/mflt/index.asp) | RRID:SCR_016106 | |
| Software, algorithm | ImageJ | ImageJ (http://imagej.nih.gov/ij/) | RRID:SCR_003070 | |
| Software, algorithm | GraphPad Prism | GraphPad (https://www.graphpad-prism.cn/) | RRID:SCR_002798 | Version 9 |