Abstract
Three different types of subcutaneous tissue cages were evaluated as sources of tissue fluid in cattle. The biochemical composition of fluid sampled at various intervals after insertion was analysed and compared to corresponding serum values. It was shown that steel netting cages were quickly obliterated by ingrowing tissue and therefore unsuitable. The two types of silicone rubber tubing cages, on the other hand, were not obliterated and could be sampled repeatedly for volumes of about 2 ml tissue cage fluid (TCF) up to at least 32 weeks after insertion. Repeated sampling in a 12 h period was also possible. The levels of total protein and albumin in TCF were lower than in serum, and were shown to decline in the first 12 weeks after insertion to a level of about 35% of the serum concentration. Calcium and magnesium levels were also lower in TCF as compared to serum. The levels of chloride and potassium were slightly higher in TCF than in serum. For sodium no difference was observed and the results for inorganic phosphorous were not uniform. Repeated sampling did not alter the total protein and albumin level in TCF. It was also shown that cages with larger open areas had lower total protein content.
Keywords: diagnostic test, sensitivity, serum enzymes, liver function, canine
Sammanfattning
Tre olika typer av subkutan a vävnadskammare som källa för vävnadsvätska från nötkreatur har utvärderats. Den biokemiska sammansättningen av vätska samlad vid olika tidpunkter efter implantation av kamrarna har analyserais och jämförts med samtidiga serumnivaer. Stalnätskammare visade sig vara olämpliga da de snabbt igensattes av inväxande vävnad. De 2 typerna av silikongummikammare künde däremot användas i minst 32 veckor. Provvolymer upp tili 2 ml vävnadsvätska künde samlas vid varje provtagningstillfälle. Upprepad provtagning under en 12-timmarsperiod var också möjlig. Nivåerna av totalprotein och albumin var lägre i vävnadsvätska än i serum och sjönk under de första 12 veckorna efter implantationen till en nivå av ungefär 35 % av serumkoncentrationen. Även kalcium och magnesium hade lägre nivåer i vävnadsvätska an i serum. Klorid och kalium hade något högre koncentrationer i vävnadsvätska an i serum medan ingen skillnad sågs för natrium. Oorganiskt fosfor gav varierande koncentrationer. Upprepad provtagning under en 12-timmarsperiod påverkade inte totalprotein och albuminnivåerna i vävnadsvätska. Koncentrationen av totalprotein var större i kammare med större öppen y ta.
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Footnotes
This investigation was supported by the Swedish Council for Forestry and Agricultural Research.
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