Abstract
Growth of mastitis pathogenic bacteria was measured in bovine whey samples by a turbidometric microtechnique. Whey from mastitis cows supported growth as compared with whey prepared from normal milk. Blood proteins leak into milk during mastitis. A study was undertaken to analyze which molecules from blood would promote bacterial growth in whey Fractions containing hemoglobin showed a distinct growth-promoting effect. An inadequate iron supply is one of the restricting growth factors for bacteria in milk. By utilizing heme-compounds the pathogens can by-pass the effect of antimicrobial iron-binding present in milk in the form of lactoferrin.
Keywords: milk, bovine milk, bovine mastitis, milk bacteria, heme, hemoglobin
Sammendrag
Tillväxt av mastitpatogena bakterier framkallades i prover av kovassla med turbidometrisk mikroteknik. Jämfört med vassla från normal mjölk förbättrade vassla från mastitkor bakterietillväxten. Vid mastit läcker blodproteiner ut i mjölken. Man analyserade vilka molekyler från blodet hade en tillväxtfrämjande effekt i vasslan. Fraktioner innehållande hemoglobin hade en markant tillväxtbefrämjande effekt. En inadekvat tillgång på järn är en av de tillväxthämmande faktorerna för bakterier i mjölk. Genom att använda hem-föreningar kan patogenerna kringgå den antimikrobiella järnbindande effekten i mjölk i form av laktoferrin.
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