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. 1984 Sep 1;25(3):352–364. doi: 10.1186/BF03547250

Detection of Chloramphenicol Residues in Pigs with Different Agar Diffusion Methods

Undersökning av kloramfenikolrester hos gris med olika agardiffusionsmetoder.

Hannu Korkeala 1,✉, Osmo Mäki-Petäys 1
PMCID: PMC8287482  PMID: 6441473

Abstract

Various tissues and body fluids of pigs given chloramphenicol intramuscularly at a dose level of 20 mg/kg 1.5, 2.5 and 18 h before slaughter were examined for drug residues with different agar diffusion methods. Zones of inhibition were observed in bile, kidney, muscle, serum and urine samples 1.5 h after drug administration. After 19 h, residues were found only in the urine. The treatment of the bile, kidney, serum and urine samples with β-glucuronidase lowered the detection threshold of the agar diffusion methods for chloramphenicol. In addition, β-glucuronidase can be used for the identification of chloramphenicol residues. Chloramphenicol yielded the greatest zones of inhibition in kidney medulla and especially in urine with and without β-glucuronidase. 18 h after drug administration residues were found with β-glucuronidase treatment only in these samples. Urine and kidney medulla proved the best samples in the residue analysis of chloramphenicol at meat inspection.

Keywords: luteolysis, hypocalcaemia

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