Abstract
An experiment concerning 6 different vitamin E treatments was conducted with 30 young pigs. From 4 to 15 weeks of age the pigs were kept in individual pens and fed a selenium supplemented basal diet consisting mostly of propionic acid treated barley and soybean meal, and containing 4.4 mg vitamin E per kg. The treatments were periods with or without vitamin E supplement (20 mg/kg) or a vitamin E injection (200 mg). Blood samples collected during the period of investigation were examined for vitamin E and for resistance against erythrocyte lipid peroxidation (ELP) in order to evaluate the antioxidant status.
Analysis of variance showed a litter effect on ELP values at all the weekly investigations and a treatment effect from two weeks after the experiment had started. Also the blood vitamin E level was litter dependent and influenced by treatment. Paired comparisons by Student’s t-test showed a delay of 1 to 2 weeks in the effect on ELP of a dietary vitamin E supplement. In contrast, both ELP and vitamin E changed very rapidly (hours) after vitamin E injections.
Independently of the vitamin E treatments there was a rise in ELP within the first 2 or 3 weeks after weaning; this was taken as an index of a reduced antioxidant status during that period.
Key words: Vitamin E, antioxidant status, erythrocyte lipid peroxidation, pigs
Sammendrag
Ialt 30 grise blev i perioden fra de blev fravænnet 4 uger gamle og til de var 15 uger garnie holdt i enkeltstier og fodret med et selen-beriget grundfoder hovedsagelig bestående af propionsyrebehandlet byg og soyaskrå og indeholdende 4,4 mg α-tocopherol pr. kg. Der blev anvendt 6 forsøgsbehandlinger i form af vitamin-E injektion eller forskellige perioder med et vitamin-E tilskud på 20 mg α-tocopherol-acetat pr. kg foder. Blodprøver udtaget 2 gange i forsøgsperioden blev undersøgt for vitamin-E indhold i plasma, ligesom blodlegemernes resistens mod peroksydering (ELP) blev besternt ugentlig for herved at vurdere grisenes antioxydantstatus.
Ved variansanalyse fandtes en konstant kuldeffekt på ELP-vser-dierne, ligesom disse var afhsengige af forsøgsbehandlingerne fra 2 uger efter forsøgets start og forsøgsperioden ud. Også blodets vitamin-E indhold var influeret af kuld og forsøgsbehandling. Ved parrede sammenligninger (t-test) fandtes ELP først at aendre sig signifikant 1–2 uger efter at et vitamin-E-tilskud til foderet var påbegyndt. Når vitamin-E blev givet som injektionsbehandling, skete ELP-a3ndringerne i løbet af få timer.
Uafhaengig af ora grisene fik vitamin-E-tilskud eller ej fandtes der en nedsat resistens mod peroksydering (forhøjede ELP værdier) i de første 2 til 3 uger efter forsøgets start (fravænningsperioden) som tegn på en nedsat antioxydantstatus i denne periode.
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