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. 1983 Dec 1;24(4):362–373. doi: 10.1186/BF03546710

Plaque Assay of Bovine Rotavirus

Plaque titrering af bovin rotavirus

G Butchaiah 1, E Lund 1,✉
PMCID: PMC8291256  PMID: 6326542

Abstract

Incorporation of trypsin and diethylaminoethyl-dextran in the overlay was found to be necessary for infectivity assay of the UK strain of bovine rotavirus by plaque assays. Small plaques of about 1 mm in radius were formed in BGM cells. Large plaques of about 3–4 mm in radius were consistently produced in monolayers of secondary calf kidney cultures.

Key words: bovine rotavirus, plaque assay, trypsin, diethyl-aminoethy1-dextran

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