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. 2020 Jul 14;11(1):769–778. doi: 10.1080/21655979.2020.1791590

Figure 5.

Figure 5.

Accelerated DSB repair in MUC1-overexpressing cells after IR. (a) NC and MUC1 transfected cells were irradiated with 2 Gy and immunostained for 53BP1 (green) and phospho-γH2AX (red) foci at 24 h after radiation. (b) NC shRNA and MUC1 shRNA transfected cells were irradiated with 2 Gy and immunostained for 53BP1 (green) and phospho-γH2AX (red) foci at 24 h after radiation. Colocalized foci (yellow) were counted at 24 h (average, 50 nuclei). DNA repair kinetics between these two cells was obtained by plotting the percentage of remaining foci against time.