Fig. 3.

Linalool suppresses HDMEC tube formation and spheroid sprouting. a Phase-contrast microscopic images of tube-forming HDMECs. The cells were exposed for 18 h to 0.25, 1 and 2 mM linalool or vehicle (0 mM). Scale bar 750 µm. b Tube formation (in % of 0 mM) of HDMECs, which were treated with vehicle or linalool, as assessed by the tube formation assay (n = 4). c Phase-contrast microscopic images of HDMEC spheroids, which were treated for 24 h with 0.25, 1 and 2 mM linalool or vehicle (0 mM). Scale bar 90 µm. d Sprouting (in % of 0 mM) of HDMEC spheroids, which were treated with vehicle or linalool, as assessed by the spheroid sprouting assay (n = 15). Means ± SEM. *P < 0.05 vs. 0 mM