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. 1982 Sep 1;23(3):325–335. doi: 10.1186/BF03546784

An Improved Medium for Culture of Mycoracterium Paratuberculosis from Bovine Faeces

Et forbedret substrat til dyrkning af Mycobacterium paratuberculosis fra kvseggødning

J Berg Jørgensen 1,✉
PMCID: PMC8295813  PMID: 7180779

Abstract

Löwenstein-Jensen medium with mycobactin, cyclo-heximide, penicillin, and chloramphenicol, and enriched with sodium pyruvate, was compared with an ordinary L.-J. medium with mycobactin. Faeces samples from cattle experimentally infected with M. paratuberculosis were cultured on both media. The improved medium gave 11 % more positive cultures and 90 % more colonies. Of the positive cultures 97 % showed detectable growth after 8 weeks of incubation, and the contamination rate was reduced to 0.4 %. By culture of faeces samples from naturally infected cattle the improved medium identified 23.2 % more infected animals than the basic medium, mostly due to a reduction of the contamination rate to about 3 %.

Keywords: Mycobacterium paratuberculosis, culture, media, cattle

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Footnotes

This work was supported by a grant from the Danish Agricultural and Veterinary Research Council.

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