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. 1982 Dec 1;23(4):515–527. doi: 10.1186/BF03546770

Procedure for Blood Glutathione Peroxidase Determination in Cattle and Swine

Analyseprocedure til bestemmelse af glutathion peroxidase i kvæg-og svineblod.

N Agergaard 1,✉, P Thode Jensen 1
PMCID: PMC8295817  PMID: 7168428

Abstract

An improved testing system has been developed for direct measurement of glutathione peroxidase activity in heparinized whole blood at 37°C. Without loss in net yield the consumption of reagents has been found to be considerably lower with the new technique than with previously described techniques. Within the range 0–700 mKat/1 the GSH-Px activity in red cells may be measured with a high degree of accuracy and reproducibility without preceding separation and washing. The stability of the enzyme in bovine and porcine whole blood at 22°C, 4°C, and —20°C was determined.

Keywords: glutathione peroxidase, selenium status, methodology, storage, cattle, swine

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