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. Author manuscript; available in PMC: 2022 Jul 21.
Published in final edited form as: Neuron. 2021 Jun 15;109(14):2275–2291.e8. doi: 10.1016/j.neuron.2021.05.020

Figure 3. Increased synaptic pruning and phagocytosis by C9orf72−/− microglia.

Figure 3.

(A) Confocal images showing synaptophysin expression in C9orf72+/+ and C9orf72−/− cortical neuron cultures at 14 days in vitro. Scale bars: 20μm and (B) quantification, mean ±SEM, Unpaired t-test with Welch’s correction. (C) Representative confocal images of presynaptic (Bassoon and vGLUT1) and (D) postsynaptic (Homer and PSD95) markers in neuronal cultures from C9orf72+/+ and C9orf72−/− mice. Scale bars: 10μm. (E-H) Quantification of pre- and post-synaptic marker densities. (I) Representative 3D confocal images of synaptophysin density around C9orf72+/+ and C9orf72−/− Cd11b+ microglia. Inset shows synaptophysin+ puncta inside Cd11b microglia. Scale bars: 20μm. (J) Quantification of synaptophysin density, Two-way ANOVA with Sidak’s multiple comparison test, mean ±SEM, *p<0.05, **p<0.005. (K) Number of synaptophysin puncta inside microglia, mean ±SEM, n=10 cells per genotype, Unpaired t-test, *p<0.05. White arrows indicate synaptophysin puncta inside microglia and is zoomed in white box.