Abstract
The effect of molybdenum (Mo) and copper (Cu) supplementation on some blood parameters and trace elements of tissues of cocks was investigated. The animals were fed with commercial poultry feed and water ad libitum and the experimental groups received peroral supplementation of different amounts of Mo or Cu during 4 weeks. Lowered values of serum uric acid were established in animals receiving 400 µg supplementation of either Mo or Cu. In contrast, the cocks receiving 100 µg supplementation of Cu displayed elevated concentrations of uric acid in the serum. A very significant statistical difference was noted between the uric acid levels of the animals receiving either moderate (group IV) or excess (group V) supplementation of Cu. The cocks with an excess of Cu displayed lowered hemoglobin and hematocrit values but no signs of Cu-intoxication were found in macro- and microscopical studies. Atomic absorption spectrophotometry was employed in the determinations of the content of Mo, Cu, Zn, Mn, Fe and & in the liver and kidneys of the cocks. The authors suggest that the results of the trace element analyses presented are to be considered as preliminary values. Furthermore, the effect of Mo and Cu on uric acid metabolism should be additionally clarified by applying histochemical studies on xanthine oxidase in different tissues of cocks in order to make conclusions on the significance of Mo and Cu in the etiology of avian gout.
Keywords: molybdenum, copper, uric acid, poultry
Sammanfattning
Effekten av molybden (Mo) och koppar (Co) tillskottet på ett antal blodpar ametrar och vävnaders spårämnes halt hos tuppar undersöktes. Djuren utfodrades med kommersiellt hömsfoder och vatten ad libitum och experimentgruppema erhöli peroralt särskilda Mo och Gu tillskott under 4 veckor. Sänkta värden av serums urinsyra noterades hos djur, som erhöli ett 400 μg stort tillskott anitingen av Mo eller Cu. Däremot uppvisade tupparna, som erhöli ett Cu tillskott på 100 μg ökad konsenitration av urinsyra i serumet. Statistiskt mycket signifikant skillnad noterades melian urinsyra nivåerna hos djuren, som erhöli antingen moderat eller ökat tillskott av Cu. Tupparna med överskott av Cu uppvisade sänkta hemoglobin och hematokrit värden, men inga tecken på Cu förgiftning kunde påvisas medelst makro- och mikroskopiska undersökningar. Atomabsorption spektrofotometri användes för bestämning av halten av Mo, Gu, Zn, Mn, Fe och Se i tupparnas lever och njurar. Författarna antyder att resultaten av spårämnes analyserna i tabell 2 måste beaktas som preliminära värden. Dessutom måste verkan av Mo och Cu på urinsyreämnesomsättningen ytterligare utredas genom att bruka histokemiska umdersökningar av xantin oxidas i olika vävnader hos tuppar för att dra slutsatser om Mo:s och Cu:s betydelse i giktens etiologi hos fåglar.
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