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. 2021 Jan 23;22(3-4):472–479. doi: 10.1186/BF03548672

Effects of Different Anticoagulants on Determination of Erythrocyte Glutathione Peroxidase

Effekt av olika antikoagulantia på beståmning av glutathionperoxidas i erytrocyter

Kamal S M Hussein 1, Bernt-E V Jones 1,✉
PMCID: PMC8300441  PMID: 6807069

Abstract

Glutathione peroxidase (GSH-Px) was determined in whole blood from cows, goats and horses using cumenehydroperoxide as substrate. Heparin was found to be the most suitable anticoagulant. The highest activities of GSH-Px were found with high concentrations of heparin in the blood samples (1000 and 1250 IU/ml of blood). Sodium fluoride and especially EDTA and sodium citrate gave lower activities of the enzyme.

Storage of the blood samples at room temperature (~20°C) or in a refrigerator (~5°C) for 3 days resulted in significantly lower activities of the enzyme, especially in horse blood.

Keywords: glutathione peroxidase, anticoagulants, storage, cattle, horse, goat

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