Abstract
Feces samples from swine-herds with severe problems of neonatal diarrhoea, 3 weeks scours or early weaning diarrhoea were examined for porcine rotavirus using three techniques: EM, ELISA and CIET. Infection with rotavirus was found in about one third of the feces samples, representing more than half of the examined swine-herds. EM and ELISA revealed nearly all the samples found to be positive, while CIET as used here detected only half of them.
Keywords: porcine rotavirus, EM, GIET, ELISA, diarrhoea
Sammendrag
Fæcesprøver fra svinebesætninger med længere varende problemer med neonatal, tre ugers eller tidlig fravænnings diarré blev undersøgt for tilstedevserelse af porcin rotavirus ved hjælp af elektronmikroskopi (EM), “enzyme linked immunosorbent assay” (ELISA), og modstrømselektroforese (CIET). Rotavirus blev påvist i omkring en trediedel af fæcesprøverne og i mere end halvdelen af de undersøgte svinebesætninger. I næsten alle rotavirus-positive prøver fandtes virus ved hjælp af EM og ELISA, mens det kun var muligt at påvise virus i halvdelen af alle rotavirus-positive prøver ved hjælp af CIET.
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Footnotes
This work was supported by the Danish Agricultural and Veterinary Research Council.
References
- Askaa J., Sørensen K. J., Moslet U. Rotavirus som årsag til diarré hos grise. (Rotavirus as a cause of diarrhoea in piglets). Dansk Vet.-T. 1980;63:730–735. [Google Scholar]
- Bachmann P. A. Rotavirusnachweis in Faezes: Erfahrungen mit dem Enzyme Linked Immunosorbent Assay (ELISA) (Demonstration of rotavirus in faeces: Experiences with the enzyme linked immunosorbent assay (ELISA)). Zbl. Vet.-Med. B. 1979;26:835–842. [PubMed] [Google Scholar]
- Bachmann P. A., Hess R. G., Hänichen T. Isolierung und Identifizierung von Rotaviren als Durchfallerreger bei Ferkeln und deren Verbreitung beim Schwein in der Bundesrepublik Deutschland. (Isolation and identification of rotavirses as cause of diarrhoea in piglets and their distribution in pigs in Germany). Tierärztl. Umsch. 1979;34:825–828. [Google Scholar]
- Bohl E. H., Kohler E. M., Saif L. J., Cross B. F., Agnes A. G., Theil K. W. Rotavirus as a cause of diarrhea in pigs. J. Amer. vet. med. Ass. 1978;172:458–463. [PubMed] [Google Scholar]
- Chasey D., Lucas M. Detection of rotavirus in experimentally infected piglets. Res. Vet. Sci. 1977;22:124–125. doi: 10.1016/S0034-5288(18)33326-5. [DOI] [PubMed] [Google Scholar]
- Corthier, G., P. Vannier, F. Gosselin & J. P. Tillon: Rotavirus in France: Serological surveys, virus isolation and experimental infection. Proc. Int. Pig Vet. Soc. Congr., Copenhagen 1980, p. 127.
- Debouch P., Pensaert M. Experimental infection of pigs with Belgian isolates of the porcine rotavirus. Zbl. Vet.-Med. B. 1979;26:517–526. doi: 10.1111/j.1439-0450.1979.tb00845.x. [DOI] [PubMed] [Google Scholar]
- Ellens D. J., de Leeuw P. W. Enzyme-linked immunosorbent assay for diagnosis of rotavirus infections in calves. J. clin. Microbiol. 1977;6:530–532. doi: 10.1128/jcm.6.5.530-532.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ellens D. J., de Leeuw P. W., Straver P. J., van Balken J. A. M. Comparison of five diagnostic methods for the detection of rotavirus antigens in calf faeces. Med. Microbiol. Immunol. 1978;166:157–163. doi: 10.1007/BF02121145. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Grauballe P. C., Genner J., Meyling A., Hornsleth A. Rapid diagnosis of rotavirus infections: Comparison of electron microscopy and immunoeleotroosmophoresis for the detection of rotavirus in human infantile gastroenteritis. J. gen. Virol. 1977;35:203–218. doi: 10.1099/0022-1317-35-2-203. [DOI] [PubMed] [Google Scholar]
- Grauballe P. C., Meyling A., Genner J. Immunoelectrophoretic studies of rotaviruses: Preparation of rabbit antibodies to imrauno-precipitates of human rotavirus and their use for identification of specific and common antigens of human and bovine rotavirus. Inserm. 1979;90:413–428. [Google Scholar]
- Grauballe, P. C., B. F. Vestergaard, A. Meyling & J. Genner: Optimized enzyme-linked immunosorbent assay for detection of human and bovine rotavirus in stools: Comparison with electron-microscopy, immunoelectro-osmophoresis and fluorescens antibody techniques. J. med. Virol. 1981, in press. [DOI] [PubMed]
- Gregory, D. W. & B. J. S. Pirie: Wetting agents for electron microscopy of biological specimens. Proc. 5th Europ. Gongr. Electron. Microsc. 1972, p. 234–235.
- Kapikian A. Z., Cline W. L., Kim H. W., Kalica A. R., Wyatt R. G., van-Kirk D. H., Chanock R. M., James D. H., Vauglin A. L. Antigenetic relationships among five reovirusrlike (RVL) agents by complementfixation (GF) and development of new substitute CF antigens for the human RVL agent of infantile gastroenteritis. Proc. Soc. exp. Biol. (N.Y.) 1976;152:535–539. doi: 10.3181/00379727-152-39434. [DOI] [PubMed] [Google Scholar]
- Lecce J. G., King M. W., Mock R. Reovirus-like agent associated with fatal diarrhea in neonatal pigs. Infect. Immun. 1976;14:816–825. doi: 10.1128/IAI.14.3.816-825.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McNulty M. S., Pearson G. R., McFerran J. B., Collins D. S., Allan G. M. A reovirus-like agent (rotavirus) associated with diarrhoea in neonatal pigs. Vet. Microbiol. 1976;1:55–63. doi: 10.1016/0378-1135(76)90009-2. [DOI] [Google Scholar]
- Middleton P. G., Petrie M., Hewitt C. M., Szymanski M. T., Tam J. S. Counter immunoelectrophoresis for the detection of infantile gastroenteritis virus (orbi-group) antigen and antibody. J. clin. Path. 1976;29:191–197. doi: 10.1136/jcp.29.3.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rodger S. M., Craven J. A., Williams I. Demonstration of reovirus-like particles in intestinal contents of piglets with diarrhoea. Aust. vet. J. 1975;51:536. doi: 10.1111/j.1751-0813.1975.tb06917.x. [DOI] [PubMed] [Google Scholar]
- Saif L. J., Bohl E. H., Kohler E. M., Hughes J. H. Immune electron microscopy of transmissible gastroenteritis virus and rotavirus (reovirus-like agent) of swine. Amer. J. vet. Res. 1977;38:13–20. [PubMed] [Google Scholar]
- Spence L., Fauvel M., Bouchard S. S., Babiuk L., Saunders J., Jr. Test for reovirus-like agent. Lancet. 1975;2:322. doi: 10.1016/S0140-6736(75)92752-X. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Theil K. W., Bohl E. H., Agnes A. G. Cell culture propagation of porcine rotavirus (reovirus-like agent) Amer. J. vet Res. 1977;38:1765–1768. [PubMed] [Google Scholar]
- Theil K.W., Bohl E. H., Cross R. F., Kohler E. M., Agnes A. G. Pathogenesis of porcine rotaviral infection in experimentally inoculated gnotobiotic pigs. Amer. J. vet. Res. 1978;39:213–220. [PubMed] [Google Scholar]
- Tzipori S., Williams I. H. Diarrhoea in piglets inoculated with rotavirus. Aust. vet. J. 1978;54:188–192. doi: 10.1111/j.1751-0813.1978.tb02447.x. [DOI] [PubMed] [Google Scholar]
- Woode G. N., Bridger J., Hall G. A., Jones J. M., Jackson G. The isolation of reovirus-like agents (rotaviruses) from acute gastroenteritis of piglets. J. med. Microbiol. 1976;9:203–209. doi: 10.1099/00222615-9-2-203. [DOI] [PubMed] [Google Scholar]