Abstract
Using a simple in vitro test it was demonstrated that staphylococci, Streptococcus agalactiae, and micrococci, the species of bacteria which are commonly isolated from udder infections, adhered to mammary gland epithelial cells readily and in large numbers. Some strains of organisms which are associated with sporadic outbreaks or occur less commonly, like Str. dysgalactiae and Str. uberis, adhered moderately. Escherichia coli, Klebsiella spp., Corynebacterium pyogenes, C. bovis, Str. bovis, and Str. faecalis, species which are isolated occasionally, adhered poorly. From these studies, it appears that selective adherence of bacteria to the epithelial cells is a factor contributing to the ability of organisms to infect the mammary gland and may, therefore, be considered an important stage in the pathogenesis of bovine mastitis.
Keywords: bovine mammary gland, mastitis, adherence, staphylococci, streptococci
Sammanfattning
Genom att använda en enkel in vitro test kan man demonstrera hur stafylokocker, Str. agalactiae, mikrokocker och andra bakterier som ofta påträffas vid bovin mastit „fastnar“ på juverepitelceller. Antalet bakterier som fastnar på epitelcellarna varierar emellertid. Bakterier av de förutnämnda arterna fastnar ofta i stort antal. Bakterier som ses vid mer sporadiskt inträffade mastiter (t ex Str. dysgalactiae och Str. uberis) fastnar däremot i mindre antal. E. coli, Klebsiella, C. pyogenes, C. bovis, Str. bovis och Str. faecalis slutligen fastnar endast i ringa antal. Det tycks som om den selektiva adhesionen är en betydelsefull faktor för bakteriens förmåga att framkalla mastit. Den kan därför tänkas bidraga tili en ökad förståelse av den bovina mastitens patogenes.
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References
- Black R. T., Marshall R. T., Bourland C. T. Locus of mammary gland infections of Corynebacterium bovis. J. Dairy Sci. 1972;55:413–416. doi: 10.3168/jds.S0022-0302(72)85508-5. [DOI] [PubMed] [Google Scholar]
- Dodd F. H., Westgarth D. R., Griffin T. K. Strategy of mastitis control. J. Amer. vet. med. Ass. 1977;170:1124–1125. [PubMed] [Google Scholar]
- Frost A. J. Selective adhesion of microorganisms to the ductular epithelium of the bovine mammary gland. Infect. Immun. 1975;12:1154–1156. doi: 10.1128/IAI.12.5.1154-1156.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Frost A. J., Wanasinghe D. D., Woolcock J. B. Some factors affecting selective adherence of microorganisms in the bovine mammary gland. Infect. Immun. 1977;15:245–253. doi: 10.1128/IAI.15.1.245-253.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gibbons R. J. Bacterial adherence in infection and immunity. CRG Rev. Microbiol. 1973;4:49–57. [Google Scholar]
- Gibbons, R. J.: Bacterial adherence to mucosal surfaces and its inhibition by secretory antibodies. In J. Mestecky & A. R. Lawton, eds.: The Immunoglobulin A System. Plenum Press, New York 1974, pp. 315–325. [DOI] [PubMed]
- Higgs T. M., Neaue F. K., Bramley A. J. Differences in intramammary pathogenicity of four strains of Streptococcus dysgalactiae. J. med. Microbiol. 1980;13:393–399. doi: 10.1099/00222615-13-3-393. [DOI] [PubMed] [Google Scholar]
- Ofek I., Beachey E. H., Jefferson W., Campbell G. L. Cell membrane binding properties of group A streptococcal lipoteichoic acid. J. exp. Med. 1975;141:990–996. doi: 10.1084/jem.141.5.990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ofek I., Mirelman D., Sharon N. Adherence of E. coli to human mucosal cells mediated by mannose receptors. Nature (Loud.) 1977;265:623. doi: 10.1038/265623a0. [DOI] [PubMed] [Google Scholar]
- Pattison I. H. The progressive pathology of bacterial mastitis. Vet. Rec. 1958;70:114–116. [Google Scholar]
- Schwan, O. & O. Holmberg: Heifer mastitis and dry-cow mastitis: a bacteriological survey in Sweden. Vet. Microbiol. 1978/79, 3, 213–226.
- Smith, H.: The little known determinants of microbial pathogenicity. In Smith, H. & J. H. Pearce, eds.: Microbial Pathogenicity in Man and Animals. Cambridge University Press, Cambridge 1972, pp. 1–24.
- Sørensen G. Høi. Studies on the aetiology and transmission of summermastitis. Nord. Vet.-Med. 1974;26:122–132. [PubMed] [Google Scholar]
- Wanasinghe, D. D.: The role of bacterial adherence in the pathogenesis of bacterial mastitis. Ph.D. Thesis, University of Queensland, Australia, 1977.
- Wanasinghe D. D. In vitro adherence of Staphylococcus aureus to bovine mammary gland epithelial cells. Acta vet. scand. 1981;22:99–108. doi: 10.1186/BF03547211. [DOI] [PMC free article] [PubMed] [Google Scholar]