Skip to main content
NCBI home page
Acta Veterinaria Scandinavica logoLink to Acta Veterinaria Scandinavica
. 2021 Jan 23;22(3-4):360–368. doi: 10.1186/BF03548661

Milk Antitrypsin Activity During Clinical and Experimental Bovine Mastitis

Antitrypsinaktivitet i mjölk vid kliniska och experimentella mastiter

T Honkanen-Buzalski 1, T Katila 1, M Sandholm 1,✉
PMCID: PMC8300512  PMID: 7344526

Abstract

Milk trypsin-inhibitor levels were followed during clinical and experimental endotoxin-induced mastitis. Milk trypsin-inhibitor level proved to be a good indicator of mastitis, closely relating to milk BSA values. The trypsin-inhibitor pattern in the quarter milk samples during mastitis closely resembled the pattern of BSA, which indicates that the trypsin-inhibitor is blood derived and diffuses to the mammary gland lite albumin. The timing of the increase in the trypsin-inhibitor into the endotoxin infused quarter closely matched with the increase in body temperature, heart rate, decrease in blood leucocytes and increase in somatic cells. Serum zinc and iron showed a decrease a few hours later.

Keywords: trypsin-inhibitors, proteinase inhibitors, bovine mastitis, milk, milk proteins, somatic cell count, antitrypsin, endotoxin, serum zinc, serum iron, bovine serum albumin

Full Text

The Full Text of this article is available as a PDF (1.0 MB).

References

  1. Andresen Ø, Amrad J, Grøholt L E, Heiland G, Schie K A, Sylliås G A. Total thyroxine and free thyroxine index of dairy cows in relation to strength of heat. Acta vet. scand. 1980;21:108–112. doi: 10.1186/BF03546905. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Atinmo T, Baldijao C, Pond W G, Barnes R H. The effect of dietary protein restriction on serum thyroxine levels of pregnant or growing swine. J. Nutr. 1978;108:1546–1553. doi: 10.1093/jn/108.9.1546. [DOI] [PubMed] [Google Scholar]
  3. Bakke H, Tveit B. Serum levels of thyroid hormones in lines of pigs selected for rate of gain and thickness of backfat. Acta agric. scand. 1977;27:41–44. doi: 10.1080/00015127709435106. [DOI] [Google Scholar]
  4. Benjaminsen E, Karlberg K. Blodprøvetaking av gris. (Blood sampling in the pig.) Norsk Vet.-T. 1979;91:15–17. [Google Scholar]
  5. Benjaminsen E, Karlberg K. Post weaning oestrus and luteal function in primiparous and pluriparous sows. Res. Vet. Sci. 1981;30:318–322. doi: 10.1016/S0034-5288(18)32550-5. [DOI] [PubMed] [Google Scholar]
  6. Brenner K V, Pethes G, Gürtler H, Losonczy S, Grün E. Thyroxin-und Trijodthyroninkonzentration im Blutplasma von Schweinefeten und neugeborenen Ferkeln. (Thyroxine and triiodothyronine plasma concentrations in sows and newborn pigs.) Endokrinologie. 1980;75:20–28. [PubMed] [Google Scholar]
  7. Crooks J, Tulloch M I, Turnbull A C, Snædal G. Comparative incidence of goitre in pregnancy in Iceland and Scotland. Lancet. 1967;2:625–627. doi: 10.1016/S0140-6736(67)90679-4. [DOI] [PubMed] [Google Scholar]
  8. Cunha, T. J.: Mineral requirements of the pig. In Swine Feeding and Nutrition. Academic Press, New York 1977, pp. 33–73.
  9. Gershengorn, M. C. D. Glinoer & J. Robbins: Transport and metabolism of thyroid hormones. In The Thyroid Gland. Ed.: M. De Visscher. Raven Press, New York 1980, pp. 81–121.
  10. Ingram, D. L. & M. J. Dauncey: Effects of dietary composition on energy metabolism and rate of utilization of thyroxine. In Energy Metabolism. Ed.: L. E. Mount. Butterworths, London 1980, pp. 411–415.
  11. Isherwood K M, Cross B A. Effect of the suckling stimulus on secretion of prolactin and luteinizing hormone in conscious and anaesthetized rats. J. Endocr. 1980;87:437–444. doi: 10.1677/joe.0.0870437. [DOI] [PubMed] [Google Scholar]
  12. Kallfelz F A, Erali R P. Thyroid function tests in domesticated animals: Free Thyroxine Index. Amer. J. vet. Res. 1973;34:1449–1451. [PubMed] [Google Scholar]
  13. Kruse V. Production and evaluation of high-quality thyroxine anti-sera for use in radioimmunoassay. Scand. J. clin. Lab. Invest. 1976;36:95–101. doi: 10.3109/00365517609068024. [DOI] [PubMed] [Google Scholar]
  14. Larsen P R, Dockaloua J, Sipula D, Wu F M. Immunoassay of thyroxine in unextracted human serum. J. clin. Endocr. 1973;37:177–182. doi: 10.1210/jcem-37-2-177. [DOI] [PubMed] [Google Scholar]
  15. Lennon HD, Mixner J P. Relationships between plasma protein-bound iodine and certain measures of reproductive and lactational performance in dairy cattle. J. Dairy Sci. 1959;42:327–332. doi: 10.3168/jds.S0022-0302(59)90569-7. [DOI] [Google Scholar]
  16. Mauget, R.: Seasonal reproductive activity of the European wild boar. Comparison with the domestic sow. In Environmental Endocrinology. Eds: I. Assenmacher & D. S. Farner. Springer-Verlag, Berlin 1978, pp. 79–80.
  17. Miller J K, Swanson E W, Spalding G E. Iodine absorption, excretion, recycling and tissue distribution in the dairy cow. J. Dairy Sci. 1975;58:1578–1593. doi: 10.3168/jds.S0022-0302(75)84753-9. [DOI] [PubMed] [Google Scholar]
  18. Mølmen K H. Plasma protein bound iodine (PBI) in pigs from the 4th month of age, through the first oestrous cycles and during pregnancy. Zuchthygiene. 1972;7:123–126. doi: 10.1111/j.1439-0531.1972.tb00233.x. [DOI] [Google Scholar]
  19. Nachreiner, R. F. & O. J. Ginther: Gestational and periparturient periods of sows: Serum chemical and hematologic changes during gestation. Amer. J. vet. Res. 1972a, 33, 2215–2219. [PubMed]
  20. Nachreiner, R. F. St O. J. Ginther: Gestational and periparturient periods of sows: Serum chemical, hematologic, and clinical changes during the periparturient period. Amer. J. vet. Res. 1972b, 33, 2233–2238. [PubMed]
  21. Parvizi N, Elsaesser F, Smidt D, Ellendorff F. Plasma luteinizing hormone and progesterone in the adult female pig during the oestrous cycle, late pregnancy and lactation, and after ovariectomy and pentobarbitone treatment. J. Endocr. 1976;69:193–203. doi: 10.1677/joe.0.0690193. [DOI] [PubMed] [Google Scholar]
  22. Porter J C, Nansel D D, Gudelsky G A, Foreman M M, Pilotte N S, Parker C R, Jr., Burrows G H, Bates G W, Madden J D. Neuroendocrine control of gonadotropin secretion. Fed. Proc. 1980;39:2896–2901. [PubMed] [Google Scholar]
  23. Potter J D. Hypothyroidism and reproductive failure. Surg. Gynec. Obstet. 1980;150:251–255. [PubMed] [Google Scholar]
  24. Robertson H A, King G J. Plasma concentrations of progesterone, oestrone, oestradiol-17β and of oestrone sulphate in the pig at implantation, during pregnancy and at parturition. J. Reprod. Fertil. 1974;40:133–141. doi: 10.1530/jrf.0.0400133. [DOI] [PubMed] [Google Scholar]
  25. Scanlon M F, Weetman A P, Lewis M, Pourmand M, Rodriguez-Arnao M D, Weightman D R, Hall R. Dopaminergic modulation of circadian thyrotropin rhythms and thyroid hormone levels in euthyroid subjects. J. clin. Endocr. 1980;51:1251–1256. doi: 10.1210/jcem-51-6-1251. [DOI] [PubMed] [Google Scholar]
  26. Shoda Y, Ishii T. Effects of season, pregnancy and lactation on serum thyroxine level in dairy cattle. Jap. J. zootech. Sci. 1976;47:659–664. [Google Scholar]
  27. Sutherland R L, Irvine C H G. Total plasma thyroxine concentrations in horses, pigs, cattle and sheep: Anion exchange resin chromatography and ceric-arsenite colorimetry. Amer. J. vet. Res. 1973;34:1261–1265. [PubMed] [Google Scholar]
  28. Thorsøe H. Inhibition of ovulation and changes in ovarian mucopolysaccharides induced by thyroidectomy in rabbits. Acta endocr. (Kbh.) 1962;41:441–447. doi: 10.1530/acta.0.0410441. [DOI] [PubMed] [Google Scholar]
  29. Visscher, M. De & A. Burger: Evaluation of thyroid function; Diagnostic procedures in thyroid diseases. In The Thyroid Gland. Ed.: M. De Visscher. Raven Press, New York 1980, pp. 169–214.
  30. Wilson J G. Hypothyroidism in ruminants with special reference to foetal goiter. Vet. Rec. 1975;97:161–164. doi: 10.1136/vr.97.9.161. [DOI] [PubMed] [Google Scholar]

Articles from Acta Veterinaria Scandinavica are provided here courtesy of BMC

RESOURCES