Abstract
The antibodies in serum samples from an outbreak of low-virulent hog cholera in Spielbach, West Germany, 1966, as well as serum samples from pigs inoculated with hog cholera (HG) virus and bovine viral diarrhea (BVD) virus, respectively, were examined by means of 3 different methods:
A modified direct complement fixation (GF) test,
A peroxidase-linked antibody (PLA) assay based on microplates with fixed, viral-antigen containing cells,
A neutralization assay carried out in microplates using the “chessboard” principle and read by means of the peroxidaselinked antibody (NPLA) assay.
A good correlation was found in their ability to detect the antibodies. Generally neutralizing antibodies could be found 2 weeks after inoculation. By CF and PLA antibodies could be detected at the same time or up to 2 weeks later. All sera were tested by the 3 methods against both HG viral antigen and BVD viral antigen. HC-antibodies could not be distinguished from BVD-antibodies by CF but to a certain degree by PLA. BVD-antibodies could to a certain degree be distinguished from HG-antibodies by CF but not by PLA. This means that CF and PLA together provide a good possibility for differentiation between the two types of antibodies. NPLA could to a high degree of reliability distinguish between HG-antibodies and BVD-antibodies.
Keywords: hog cholera, bovine viral diarrhea, porcine serum, antibodies, peroxidase-linked antibody, microneutralization assay
Sammendrag
Til påvisning af antistofindhold i sera fra et udbrud af lavvirulent klassisk svinepest i Spielbach, Vesttyskland i 1966, samt i sera fra grise, podet med henholdsvis svinepest (SP) virus og bovin virus diarrhea (BVD) virus blev anvendt 3 forskellige metoder:
Modificeret direkte komplementbinding (GF)
Peroxidasemærket antistof i forbindelse med fixerede virusantigenholdige celler i mikroplader (PLA)
Neutralisationstitrering udført i mikroplader efter skakbrætsprincippet og aflæst ved hjælp af peroxidasemærket antistof (NPLA).
Der blev fundet god overensstemmelse i antistofpåvisningen ved de 3 metoder. Gennemgående kunne neutraliserende antistoffer påvises 2 uger efter podning, og samtidig hermed eller senest 2 uger derefter kunne antistoffer påvises ved CF og PLA. Samtlige sera blev undersøgt overfor både SP antigen og BVD antigen. SP antistoffer kunne ikke skelnes fra BVD antistoffer ved CF men med en vis sikkerhed ved hjælp af PLA. BVD antistoffer kunne med en vis sikkerhed skelnes fra SP antistoffer ved CF, men ikke ved PLA. Det vil sige, at såfremt både CF og PLA anvendes fås en mulighed for at skelne mellem SP og BVD antistoffer. NPLA kan med stor sikkerhed skelne imellem de 2 typer af antistoffer.
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