Figure 7.

Schematic representation of the impact of DDR1 silencing/knocking out on MCF7 human breast cancer cells bioenergetics. (A) DDR1 silencing of MCF7 cells was accompanied by a reduction of ATP production (both glycoATP and mitoATP) also in the presence of IGF2, which is known to stimulate cell metabolism. A similar ATP reduction was observed in MCF7^DDR1-ve cells compared to the control cells. (B) Several key molecules involved in the regulation of both glycolysis and OxPhos were negatively affected by DDR1 silencing or knocking out. Together, the data support the notion that DDR1 targeting may negatively affect breast cancer cell bioenergetics at least partially by downregulating IR-A.