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. 2021 Jun 30;10(7):1646. doi: 10.3390/cells10071646

Figure 3.

Figure 3

Flow cytometry representative dot plots with mean cell counts ± standard deviation for (A) CD45+ leukocytes and (B) CD68+ monocytes/macrophages in blood at 1, 3 and 7 days post-trauma (dpt) in osteotomy (OST; n = 10), polytrauma (PT-C; n = 10), polytrauma + IgY (PT-IgY; n = 5 for 1 and 3 dpt; n = 4 for 7 dpt) and polytrauma + anti-HMGB1 antibody (PT-Ab HMGB1; n = 10); (C) percentage (%) of CD3−CD45+ leukocytes; (D) % CD45+CD68+ monocyte/macrophages; (E) flow cytometry gating scheme to evaluate RAGE and TLR4 surface expression on CD45+CD68+ monocytes/macrophages; (F) Mean fluorescent intensity (MFI) of CD45+CD68+RAGE+ monocytes/macrophages; and (G) MFI of CD45+CD68+TLR4+ monocytes/macrophages in blood from rats with OST, PT-C, PT-IgY and PT-Ab HMGB1 at 1, 3 and 7 days. Naïve uninjured rats were used as baseline controls (n = 5). The dotted line is the mean of cell counts from naïve rats. * p < 0.05 comparing OST, PT-C, PT-IgY and PT-Ab HMGB1 cohorts. The bar graphs represent mean, whereas error bars represent SEM.