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Semipreparative HPLC separation (for conditions, see text) with resolution of the positional isomers 6-O-β-d-glucopyranosyl-feralolide (3) (tR = 10 min) and 3′-O-β-d-glucopyranosyl-feralolide (2) (tR = 12 min) relative to their approximately 2.5:1 ratio in the A. vera drug is here reported for the first time.
