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. 2021 Jul 6;10(7):1709. doi: 10.3390/cells10071709

Figure 4.

Figure 4

Repeated OGD impaired short term inhibitory plasticity in the absence of miRNA 132/212. Normalized and averaged PPI ratios (second pulse/first pulse) plotted against inter-pulse intervals from 20 to 160 ms, comparing responses from WT slices before and after the OGD treatment protocol: (A) PPI ratios, at ascending interstimulus intervals derived from WT slices and (B) miRNA-132/212−/− slices before and after the OGD treatment protocol. (C) Comparison of PPI ratios, at ascending interstimulus intervals obtained from WT and miRNA-132/212−/− slices after the OGD treatment. (D) Representative raw fEPSP traces obtained from WT (upper traces, black) and miRNA-132/212−/− slices (lower traces, gray), before (left) and after (right) OGD treatment, applying a 20 ms inter-pulse interval paired pulse stimulation. Two-way repeated measures ANOVA revealed no statistically significant differences in the comparison of PPI ratios recorded in WT slices before and after OGD treatment (A). In contrast, PPI ratios increased significantly in miRNA-132/212−/− slices after the OGD treatment, most pronounced at the 40 ms interstimulus interval (Bonferroni’s multiple comparisons tests) (B). Two-way repeated measures ANOVA revealed a significant difference between the genotypes after the OGD treatment (C). Data from n = 7 WT and n = 6 miRNA 132/212−/− is presented as mean ± SEM. p < 0.05 was considered significant. * p < 0.05. ns: no statistical significance.