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. 2021 Jul 17;26(14):4332. doi: 10.3390/molecules26144332

Table 1.

Retention times (tR) 1 and integrated peak areas (A) of the investigated chalcones (1 and 2) and their GSH adducts 2.

pH 3 Compound tR
(E)-Chalcone		 Area Ratio 4
A315/A0 		tR
(Z)-Chalcone		 Area
(Z)-Chalcone		 tR
GSH–1		 Area
GSH–1		 tR
GSH–2		 Area
GSH–2
3.2 1 16.4 0.81 16.2 <100 13.8 4245 N/D 5 -
3.2 2 15.9 0.96 15.7 <100 11.9 3352 N/D 5 -
6.3 1 16.3 0.09 16.0 <100 13.2 16,571 N/D 5 -
6.3 2 15.8 0.21 15.5 <100 11.3 17,160 N/D 5 -
8 1 16.3 0.04 16.1 6 <100 13.3 17,419 N/D 5 -
8 2 15.7 0.08 15.5 <100 11.0 20,387 N/D 5 -

1 Retention times in minutes; 2 data refer to the average of two independent measurements at the 315 min time point; 3 pH value of the aqueous thiol solution; 4 ratio of peak areas measured at 0 and 315 min; 5 not detectable; 6 only detectable at the 15, 45, 75, 135, and 165 min time points.