Figure 14.

Cytokine levels in serum and CSF samples in ALS SOD1G93A allogenic mouse model: IL-1α, IL-10, IL-12, and KC levels in serum and CSF samples were measured in allogenic ALS SOD1G93A mice. There was a striking difference in IL-1α levels in no immunosuppression and sham surgery variants and variants in which immunosuppressive versus immunomodulatory regimens were applied. Significant differences were also seen in IL-10, IL-12, and KC levels in serum samples and IL-1α and IL-10 levels in CSF samples on day 0. No significant differences were found in IL-12 and KC levels in the course of observations. Moreover, no significant differences on the day of termination for IL-1α and IL-10 levels were found. However, it is worth emphasizing that the levels of IL-10 and IL-12 in the Tacrolimus and co-stimulatory groups were undetectable in CSF. Information about other cytokines levels from the 23-cytokine mouse panel is accessible in Supplementary Figures S1–S3. Unpaired Student’s t-test; data are presented as mean, error bars: SD; the total number of samples: n = 101, total number per variant at each time point: n = 2–6. The total number of biological replicates per group: n = 6. Abbreviations: ALS-amyotrophic lateral sclerosis; CSF-cerebrospinal fluid.