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RP-HPLC chromatogram. The green plot is the reaction mixture (reaction time: 24 h) to obtain NAMP; the red plot is purified NAM in water; the blue plot is a VCAM-binding peptide solution in water. Peak 1 (tR = 3.2 min) is related to NAMP, peak 2 (tR = 5.8 min) to NAM and peak 3 (tR = 2.0 min) to the peptide.
