Abstract
Standard lipid-lowering therapies in solid organ transplantations pose challenges due to interactions with immunosuppressants. Proprotein convertase subtilisin/kexin type 9 inhibitors (PCSK9i) represent a new class of lipid-lowering therapies with potential promise in this population. We describe PCSK9i as an efficacious and safe option for management of hypercholesterolemia in solid organ transplantations. (Level of Difficulty: Advanced.)
Key Words: dyslipidemia, proprotein convertase subtilisin/kexin type 9 inhibitors, solid organ transplantation
Abbreviations and Acronyms: ASCVD, atherosclerotic cardiovascular disease; CAV, cardiac allograft vasculopathy; CVD, cardiovascular disease; FH, familial hypercholesterolemia; LDL-C, low-density lipoprotein cholesterol; LLT, lipid-lowering therapy; PCSK9, proprotein convertase subtilisin/kexin type 9; PCSK9i, proprotein convertase subtilisin/kexin type 9 inhibitor; SOT, solid organ transplantation
Standard lipid-lowering therapies in solid organ transplantations pose challenges due to interactions with immunosuppressants. Proprotein convertase…
Solid organ transplantation (SOT), encompassing heart, kidney, liver, pancreas, and lung transplants, has revolutionized treatment of end-stage disease (1). Advances in care post-SOT have led to reduced rejection rates, improved graft survival, and patient longevity. Improved patient survival after transplantation unmasked cardiovascular disease (CVD) as the leading cause of morbidity and mortality. A particularly aggressive form of CVD in patients who have undergone transplantation is allograft vasculopathy. Among heart transplant recipients, cardiac allograft vasculopathy (CAV) manifests as a progressive form of arteriosclerosis in the transplanted heart. Although risk factors for the development of CVD after transplantation are numerous, dyslipidemia is one of the strongest, most prevalent, and eminently modifiable factors. Standard lipid-lowering therapies (LLT) pose challenges in the SOT population due to potential toxicity resulting from drug-drug interactions with immunosuppressants. Readers are referred to a more comprehensive evaluation of the precautions and monitoring parameters involved with the coadministration of LLT and immunosuppressants (1).
In 2015, a new class of LLT, proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibitors (PCSK9i), was approved for use in patients with familial hypercholesterolemia (FH) and/or established atherosclerotic CVD (ASCVD) who require additional low-density lipoprotein-cholesterol (LDL-C) lowering after standard therapy. Two large randomized controlled trials that tested PCSK9i demonstrated large reductions in LDL-C (50%–60%) and improvement in cardiovascular outcomes (2,3). Enthusiasm has grown within the transplantation community for use of PCSK9i as an adjunctive treatment in patients who have failed to achieve LDL goals with standard therapies. This is exemplified in recent reports of the use of PCSK9i among SOT recipients (4, 5, 6, 7, 8). These reports leave additional questions, for example, are these agents effective and safe in different forms of SOT? Are the pharmacokinetics of PCSK9i in cases of SOT similar to those in non-SOT patients? Do PCSK9i affect immunosuppression? This case series aims to shed light on these aspects.
Learning Objectives
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Management of dyslipidemia in solid organ transplantation patients is discussed.
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Safety and effectiveness of PCSK9i in transplant patients are highlighted.
Presentation
Our clinical case series involved a group of 12 adult SOT recipients with concurrent hypercholesterolemia who were inadequately treated with standard LLTs. At baseline, 25% of patients were not taking LLT, and more than half were deemed statin intolerant. Statin treatment was predominantly low or moderate intensity, as recommended for transplantation patients (Table 1).
Table 1.
Characteristics of Solid Organ Transplant Patients Receiving PCSK9i Therapy (N = 12)
Baseline Characteristics | |
---|---|
Age, yrs | 63 ± 11 |
Males | 4 (33.3) |
Females | 8 (66.7) |
Type of solid organ transplant | |
Heart | 9 (75.0) |
Kidney | 1 (8.3) |
Liver | 1 (8.3) |
Lung | 1 (8.3) |
Time between transplantation and injection, months | 80.5 (21.5–158.5) |
FH | 6 (50.0) |
Without known ASCVD | 2 (33.3) |
With ASCVD | 4 (66.7) |
ASCVD (includes CAD diagnosed by CACS) | 8 (66.7) |
CAD | 6 (75.0) |
Cerebrovascular disease | 0 (0.0) |
PAD | 2 (25.0) |
CAV∗ | 2 (18.2) |
Diabetes mellitus | 4 (33.3) |
Chronic kidney disease (stage 3-ESRD) | 9 (75.0) |
HIV | 1 (8.3) |
LLT use | 9 (75.0) |
High = intensity statin | 1 (8.3) |
Statin-intolerant† | 7 (58.3) |
Statin monotherapy | 1 (8.3) |
Statin + ezetimibe | 4 (33.3) |
Additional LLT | 3 (25.0) |
Clinical Characteristics | Pre-PCSK9i | Post-PCSK9i |
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LDL-C, mg/dl | 141.5 (113.5–149.0) | 57 (27–69) (change: −50% [−58% to −23%]) |
PCSK9, ng/dl | 407.5 (330.5–591.4) | 3,404.3 (2,976.7–4,863.8) (change: 932% [661% to 1,025%]) |
Cyclosporine, ng/ml‡ | 102.2 (93.4–111.0) | 64.5 (57.4–71.6) (change: −37% [−39% to 36%]) |
Tacrolimus, ng/ml | 6.8 (6.0–7.4) | 7.6 (6.1–9.8) (change: 6% [−3% to 12%]) |
Sirolimus, ng/ml‡ | 3.9 (3.9–3.9) | 2.8 (2.6–3.0) (change: −27% [−32% to −21%]) |
Rejection episodes | 0 (0.0)∗ | 0 (0.0)§ |
Hospitalizations | 2 (18.2)∗ | 1 (8.3)§ |
Emergency department visits | 3 (27.3)∗ | 1 (8.3)§ |
Infections | 3 (27.3)∗ | 3 (25.0)§ |
Progression of atherosclerosis documented by post-PCSK9i catheterization | ||
Yes | -- | 0 (0.0)§ |
No | -- | 4 (33.3)§ |
Unknown (no catheterization) | -- | 8 (66.7)§ |
New cases ASCVD | -- | 0 (0.0)§ |
New cases CAV | -- | 0 (0.0)§ |
Mortality | -- | 1 (8.3)§ |
Values are mean ± SD, n (%), or median (interquartile range), unless otherwise indicated.
ASCVD = atherosclerotic cardiovascular disease; CACS = coronary artery calcium score; CAD = coronary artery disease; CAV = cardiac allograft vasculopathy; ESRD = end-stage renal disease; FH = familial hypercholesterolemia; LDL-C = low-density lipoprotein cholesterol; LLT = lipid-lowering therapy; PCSK9 = proprotein convertase subtilisin/kexin type 9; PAD = peripheral arterial disease; Pre = pre-PCSK9 inhibitor; Post = post-PCSK9 inhibitor.
Out of 11 total patients as one patient received PCSK9i prior to transplantation.
Unable to tolerate lowest therapeutic daily dose.
Levels only drawn in 2 patients.
Events post-PCSK9i initiation and post-transplant.
Medical history
The majority of patients had undergone heart transplantation (75%), but kidney, liver, and lung transplantation recipients were also represented. One-half of the patients had FH (n = 6), 75% had ASCVD (n = 8), and 18% had mild CAV (n = 2).
Investigations
All patients were initiated on PCSK9i therapy (alirocumab [n = 2], evolocumab [n = 9], or both sequentially [n = 1]). To assess the efficacy, safety, and tolerability of PCSK9i in SOT recipients in the present cohort, data collection was performed before and after PCSK9i treatment and included measurements of plasma concentrations of LDL-C, PCSK9, and immunosuppressants. The proportion meeting immunosuppression goals (9), rates of organ rejection, hospitalizations, emergency department visits, and infections were collected. All outcomes were continuously monitored during a mean observation period of 12 months starting 6 months prior to PCSK9i initiation (pre-PCSK9i) and ending 6 months after PCSK9i initiation (post-PCSK9i).
Management
Median LDL-C levels decreased 60% from 142 mg/dl to 57 mg/dl post-PCSK9i. All patients achieved optimal LDL-C levels of ≤70 mg/dl at some point during therapy. The median plasma PCSK9 concentration was 9.3-fold increased from 408 ng/dl to 3,404 ng/dl post-PCSK9i. Median plasma levels of immunosuppressants decreased for cyclosporine (37%) and sirolimus (27%) but increased for tacrolimus (6%). However, the proportion of time within therapeutic ranges for immunosuppressants did not change post-PCSK9i compared with pre-PCSK9i. Moreover, no cases of rejection occurred post-PCSK9i. Rates of hospitalization, emergency department visits, and infections pre-PCSK91 were similar to those post-PCSK9i. Only 33% (n = 4) underwent post-PCSK9i coronary artery catheterizations, but none demonstrated progression of atherosclerosis or CAV. One death occurred as the result of respiratory complications from a newly diagnosed advanced stage adenocarcinoma of the lung and was deemed unrelated to ASCVD, use of the drug, or transplantation failure. PCSK9i was well tolerated, with 25% of patients experiencing mild and self-limiting adverse drug events consisting of injection site reactions, rhinorrhea, and nausea. No patients discontinued treatment because of adverse events.
Discussion
This report describes the efficacy, safety, and tolerability of PCSK9i in a cohort of SOT patients. As expected for this population, most patients at baseline had uncontrolled dyslipidemia perpetuated by lipid-altering effects of immunosuppressant drugs required to prevent organ rejection and concerns about possible LLT toxicity after transplantation (1).
The 9.3-fold rise in PCSK9 levels after PCSK9i therapy in this SOT cohort was comparable to the 7-fold increase previously reported in non-SOT hyperlipidemic patients (10). The 60% reduction in LDL-C was comparable to the 50% to 60% reduction observed in cardiovascular outcome trials (2,3). Compared with standard LLT, which have heightened risk for significant drug-drug interactions with immunosuppressants, medication-related toxicity, and/or reduced immunosuppression levels, PCSK9i seemed to have a favorable safety profile for lipid management in SOT patients. The present study demonstrated negligible effects of PCSK9i therapy on plasma immunosuppressant concentrations. Additionally, there were no changes in rates of organ rejection, progressive atherosclerosis, hospitalization, emergency department visits, or infection.
Although other reports of PCSK9i use in SOT recipients have been published (4, 5, 6, 7, 8), the present cohort is unique for several reasons and aims to improve the understanding of the efficacy and safety of PCSK9i in this setting. This study describes the use of PCSK9i in a diverse cohort of SOT patients, including those who underwent heart, kidney, liver, and lung transplantations, the most diverse cohort evaluated to date. Additionally, prior reports insufficiently characterized the pharmacokinetic and pharmacodynamic response to PCSK9i and its effect on immunosuppression. The present study comprehensively describes the effect of PCSK9i on both of these fronts, detailing the percentage of LDL-C reduction, post-treatment LDL-C values, LDL-C goal attainment, and plasma PCSK9 levels, as well as degree of immunosuppression by evaluating average values and percentage of goal attainment. The present results indicate that PCSK9i in SOT patients perform similarly in non-SOT patients. This report adds significant granularity to the interplay between PCSK9i and immunosuppression and contributes to the growing published reports that support use of PCSK9i among SOT recipients.
Follow-up
All surviving SOT patients remained well managed on PCSK9i therapy, with frequent (6-month) follow-up examinations in the authors’ PCSK9i clinic to assess response and tolerability.
Conclusions
These results suggest that PCSK9i are an efficacious and safe treatment for hypercholesterolemia in SOT recipients. Further studies of PCSK9i in SOT are needed to assess long-term LDL-C lowering, prevention of graft vasculopathy and ASCVD, and safety.
Footnotes
Dr. Shapiro is supported by U.S. National Institutes of Health grant K12HD043488; and is an advisor for Amarin and Esperion. Dr. Fazio is supported by NIH grant RO1 5R01HL132985-02; and is an advisor for Esperion, Amgen, Novartis, AstraZeneca, and Amarin. Dr. Duell has received institutional grants from Regeneron, RegenxBio, and Retrophin; and is a consultant for AstraZeneca, Akcea, Esperion, Regeneron, RegenxBio, and Retrophin. All other authors have reported that they have no relationships relevant to the contents of this paper to disclose.
The authors attest they are in compliance with human studies committees and animal welfare regulations of the authors’ institutions and Food and Drug Administration guidelines, or patient consent where appropriate. For more information, visit the JACC: Case Reportsauthor instructions page.
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